Anti-FTO antibody [EPR6895] (ab124892)
- Datasheet
- Specific References (8)
- Protocols
Overview
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Product nameAnti-FTO antibody [EPR6895]
See all FTO primary antibodies -
DescriptionRabbit monoclonal [EPR6895] to FTO
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivityReacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human FTO aa 450-550. The exact sequence is proprietary.
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Positive control
- Human Breast and kidney tissues; 293T, SH-SY5Y, Caco2 and BxPC3 lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Dissociation constant (KD)KD = 9.30 x 10 -13 M Learn more about KD
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Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR6895
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab124892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionDioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.
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Tissue specificityUbiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.
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Involvement in diseaseDefects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GRDDCFED) [MIM:612938]. The disease consists of a severe children multiple congenital anomaly syndrome with death by the age of 3 years. All affected individuals had postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits, and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate were also observed.
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Sequence similaritiesBelongs to the fto family.
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DomainThe 3D-structure of the Fe2OG dioxygenase domain is similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity is very low. As a consequence, the domain is not detected by protein signature databases.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 79068 Human
- Omim: 610966 Human
- SwissProt: Q9C0B1 Human
- Unigene: 528833 Human
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Alternative names
- AlkB homolog 9 antibody
- ALKBH9 antibody
- Alpha-ketoglutarate-dependent dioxygenase FTO antibody
see all
Images
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![Western blot - Anti-FTO antibody [EPR6895] (ab124892)](https://www.abcam.com/ps/products/124/ab124892/Images/ab124892-280295-ab124892KOWB.jpg)
Western blot - Anti-FTO antibody [EPR6895] (ab124892)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: FTO knockout HAP1 whole cell lysate (20 µg)
Lane 3: HEK293 whole cell lysate (20 µg)
Lane 4: MOLT4 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab124892 observed at 58 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab124892 was shown to specifically react with FTO in wild-type HAP1 cells. No band was observed when FTO knockout samples were examined. Wild-type and FTO knockout samples were subjected to SDS-PAGE. ab124892 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FTO antibody [EPR6895] (ab124892)](https://www.abcam.com/ps/products/124/ab124892/Images/FTO-Primary-antibodies-ab124892-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FTO antibody [EPR6895] (ab124892)ab124892, at 1/100 dilution, staining FTO in Paraffin-embedded Human Breast tissue by Immunohistochemistry. -
![Western blot - Anti-FTO antibody [EPR6895] (ab124892)](https://www.abcam.com/ps/products/124/ab124892/Images/FTO-Primary-antibodies-ab124892-1.jpg)
Western blot - Anti-FTO antibody [EPR6895] (ab124892)All lanes : Anti-FTO antibody [EPR6895] (ab124892) at 1/1000 dilution
Lane 1 : 293T lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : Caco2 lysate
Lane 4 : BxPC3 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 58 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FTO antibody [EPR6895] (ab124892)](https://www.abcam.com/ps/products/124/ab124892/Images/FTO-Primary-antibodies-ab124892-6.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FTO antibody [EPR6895] (ab124892)ab124892, at 1/100 dilution, staining FTO in Paraffin-embedded Human kidney tissue by Immunohistochemistry. -
![Other - Anti-FTO antibody [EPR6895] (ab124892)](https://www.abcam.com/ps/products/124/ab124892/Images/FTO-Primary-antibodies-ab124892-7.jpg)
Other - Anti-FTO antibody [EPR6895] (ab124892)
References
This product has been referenced in:
- Su R et al. R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA Signaling. Cell 172:90-105.e23 (2018). WB ; Human . Read more (PubMed: 29249359) »
- Aas A et al. Nucleocytoplasmic Shuttling of FTO Does Not Affect Starvation-Induced Autophagy. PLoS One 12:e0168182 (2017). Read more (PubMed: 28288181) »
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