Product nameAnti-FTSJD2 antibody
See all FTSJD2 primary antibodies
DescriptionRabbit polyclonal to FTSJD2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
A region between residue 800 and the C-terminus (residue 835) of human FTSJD2
- Whole cell lysate from 293T cells transfected with a myc-FTSJD2 expression construct. Whole cell lysate from 293T cells. Whole cell lysate from G361 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000 - 1/25000. Detects a band of approximately 100 kDa (predicted molecular weight: 96 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionS-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap1 2'-O-ribose methylation to the 5'-cap structure of mRNAs. Methylates the ribose of the first nucleotide of a m(7)GpppG-capped mRNA and small nuclear RNA (snRNA) to produce m(7)GpppRm (cap1). Displays a preference for cap0 transcripts. Cap1 modification is linked to higher levels of translation. May be involved in the interferon response pathway.
Sequence similaritiesContains 1 G-patch domain.
Contains 1 RrmJ-type SAM-dependent 2'-O-MTase domain.
Contains 1 WW domain.
- Information by UniProt
- Cap methyltransferase 1 antibody
- Cap-specific mRNA (nucleoside-2''-O-)-methyltransferase 1 antibody
- Cap1 2''O-ribose methyltransferase 1 antibody
All lanes : Anti-FTSJD2 antibody (ab70388) at 0.1 µg/ml
Lane 1 : Whole cell lysate from 293T cells transfected with a
myc-FTSJD2 expression construct at 15 µg
Lane 2 : Whole cell lysate from 293T cells that were
mock transfected at 60 µg
Lane 3 : Whole cell lysate from G361 cells at 15 µg
Predicted band size: 96 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
ab70388 has not yet been referenced specifically in any publications.