Anti-FTSJD2 antibody (ab70388)
Key features and details
- Rabbit polyclonal to FTSJD2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FTSJD2 antibody
See all FTSJD2 primary antibodies -
Description
Rabbit polyclonal to FTSJD2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human FTSJD2 (C terminal).
Database link: Q8N1G2 -
Positive control
- Whole cell lysate from 293T cells transfected with a myc-FTSJD2 expression construct. Whole cell lysate from 293T cells. Whole cell lysate from G361 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/25000. Detects a band of approximately 100 kDa (predicted molecular weight: 96 kDa).
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Notes |
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WB
1/5000 - 1/25000. Detects a band of approximately 100 kDa (predicted molecular weight: 96 kDa). |
Target
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Function
S-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap1 2'-O-ribose methylation to the 5'-cap structure of mRNAs. Methylates the ribose of the first nucleotide of a m(7)GpppG-capped mRNA and small nuclear RNA (snRNA) to produce m(7)GpppRm (cap1). Displays a preference for cap0 transcripts. Cap1 modification is linked to higher levels of translation. May be involved in the interferon response pathway. -
Sequence similarities
Contains 1 G-patch domain.
Contains 1 RrmJ-type SAM-dependent 2'-O-MTase domain.
Contains 1 WW domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23070 Human
- Entrez Gene: 100172922 Orangutan
- SwissProt: Q8N1G2 Human
- SwissProt: Q5R981 Orangutan
- Unigene: 520102 Human
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Alternative names
- Cap methyltransferase 1 antibody
- Cap-specific mRNA (nucleoside-2''-O-)-methyltransferase 1 antibody
- Cap1 2''O-ribose methyltransferase 1 antibody
see all
Images
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All lanes : Anti-FTSJD2 antibody (ab70388) at 0.2 µg
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 96 kDa -
All lanes : Anti-FTSJD2 antibody (ab70388) at 0.1 µg/ml
Lane 1 : Whole cell lysate from 293T cells transfected with a
myc-FTSJD2 expression construct at 15 µg
Lane 2 : Whole cell lysate from 293T cells that were
mock transfected at 60 µg
Lane 3 : Whole cell lysate from G361 cells at 15 µg
Predicted band size: 96 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab70388 has not yet been referenced specifically in any publications.