Recombinant
RabMAb

Recombinant Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

Submit a review Submit a question

Overview

  • Product name

    Anti-FUBP1/FBP antibody [EPR12326]
    See all FUBP1/FBP primary antibodies

  • Description

    Rabbit monoclonal [EPR12326] to FUBP1/FBP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human FUBP1/FBP aa 50-150. The exact sequence is proprietary.
    Database link: Q96AE4

  • Positive control

    • WB: HeLa, Jurkat and HepG2 whole cell lysate (ab7900). IHC-P: Human endometrium and ovarian adenocarcinoma tissues. ICC/IF: HeLa cells. IP: FUBP1/FBP IP in HeLa cell lysate.

  • General notes

    Previously labeled as FUBP1. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 68 kDa.
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/100.
ICC/IF 1/1000.

Target

  • Function

    Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.

  • Sequence similarities

    Contains 4 KH domains.

  • Post-translational
    modifications

    Ubiquitinated. This targets the protein for proteasome-mediated degradation.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • DNA helicase V antibody
      • far upstream element (FUSE) binding protein 1 antibody
      • Far upstream element (FUSE) binding protein 4 antibody
      • Far upstream element binding protein 1 antibody
      • far upstream element binding protein antibody
      • Far upstream element-binding protein 1 antibody
      • FBP antibody
      • FUBP antibody
      • Fubp1 antibody
      • FUBP1_HUMAN antibody
      • Fubp4 antibody
      • FUSE binding protein 1 antibody
      • FUSE-binding protein 1 antibody
      • HDH V antibody
      see all

    Images

    • Western blot - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
      Western blot - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
      All lanes : Anti-FUBP1/FBP antibody [EPR12326] (ab192867) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 68 kDa
      Observed band size: 74 kDa
      why is the actual band size different from the predicted?



      Blocking buffer and concentration: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

      Immunohistochemical analysis of paraffin embedded human endometrium tissue labeling FUBP1/FBP using ab192867 at a 1/2000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

      Immunohistochemical analysis of paraffin embedded human ovarian adenocarcinoma tissue labeling FUBP1/FBP using ab192867 at a 1/2000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
      Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

      Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling FUBP1/FBP using ab192867 at a 1/1000 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary antibody, at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.

    • Immunoprecipitation - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
      Immunoprecipitation - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

      Western blot analysis of pellet from HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate immunoprecipitated using ab192867 at a 1/100 dilution. Secondary antibody was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated, at a 1/1000 dilution.

    References

    ab192867 has not yet been referenced specifically in any publications.

    Publishing research using ab192867? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab192867.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up