RecombinantRabMAb

Recombinant Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12326] to FUBP1/FBP
  • Suitable for: WB, IHC-P, IP, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-FUBP1/FBP antibody [EPR12326]
    See all FUBP1/FBP primary antibodies

  • Description

    Rabbit monoclonal [EPR12326] to FUBP1/FBP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human FUBP1/FBP aa 50-150. The exact sequence is proprietary.
    Database link: Q96AE4

  • Positive control

    • WB: HeLa, Jurkat and HepG2 whole cell lysate (ab7900). IHC-P: Human endometrium and ovarian adenocarcinoma tissues. ICC/IF: HeLa cells. IP: FUBP1/FBP IP in HeLa cell lysate.

  • General notes

    Previously labeled as FUBP1. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab192867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 68 kDa.
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/100.
ICC/IF 1/1000.

Target

  • Function

    Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.

  • Sequence similarities

    Contains 4 KH domains.

  • Post-translational
    modifications

    Ubiquitinated. This targets the protein for proteasome-mediated degradation.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • DNA helicase V antibody
      • far upstream element (FUSE) binding protein 1 antibody
      • Far upstream element (FUSE) binding protein 4 antibody
      • Far upstream element binding protein 1 antibody
      • far upstream element binding protein antibody
      • Far upstream element-binding protein 1 antibody
      • FBP antibody
      • FUBP antibody
      • Fubp1 antibody
      • FUBP1_HUMAN antibody
      • Fubp4 antibody
      • FUSE binding protein 1 antibody
      • FUSE-binding protein 1 antibody
      • HDH V antibody
      see all

    Images

    • Western blot - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
      Western blot - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
      All lanes : Anti-FUBP1/FBP antibody [EPR12326] (ab192867) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 68 kDa
      Observed band size: 74 kDa
      why is the actual band size different from the predicted?



      Blocking buffer and concentration: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

      Immunohistochemical analysis of paraffin embedded human endometrium tissue labeling FUBP1/FBP using ab192867 at a 1/2000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

      Immunohistochemical analysis of paraffin embedded human ovarian adenocarcinoma tissue labeling FUBP1/FBP using ab192867 at a 1/2000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
      Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

      Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling FUBP1/FBP using ab192867 at a 1/1000 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary antibody, at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.

    • Immunoprecipitation - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
      Immunoprecipitation - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

      Western blot analysis of pellet from HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate immunoprecipitated using ab192867 at a 1/100 dilution. Secondary antibody was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated, at a 1/1000 dilution.

    • Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
      Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

    References (1)

    Publishing research using ab192867? Please let us know so that we can cite the reference in this datasheet.

    ab192867 has been referenced in 1 publication.

    • Han T  et al. NORAD orchestrates endometrial cancer progression by sequestering FUBP1 nuclear localization to promote cell apoptosis. Cell Death Dis 11:473 (2020). PubMed: 32555178

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