Product nameAnti-FUBP1/FBP antibody [EPR12326] (HRP)
See all FUBP1/FBP primary antibodies
DescriptionRabbit monoclonal [EPR12326] to FUBP1/FBP (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human FUBP1/FBP aa 50-150. The exact sequence is proprietary.
Database link: Q96AE4
- WB: HeLa, Jurkat and HepG2 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: 1% BSA, 30% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab209049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 77 kDa (predicted molecular weight: 68 kDa).|
FunctionRegulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.
Sequence similaritiesContains 4 KH domains.
modificationsUbiquitinated. This targets the protein for proteasome-mediated degradation.
- Information by UniProt
- DNA helicase V antibody
- far upstream element (FUSE) binding protein 1 antibody
- Far upstream element (FUSE) binding protein 4 antibody
All lanes : Anti-FUBP1/FBP antibody [EPR12326] (HRP) (ab209049) at 1/5000 dilution
Lane 1 :
HeLa whole cell lysate (ab150035)
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209049 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab209049 has not yet been referenced specifically in any publications.