Overview

  • Product name

    Anti-FUBP1/FBP antibody [EPR19208]
    See all FUBP1/FBP primary antibodies
  • Description

    Rabbit monoclonal [EPR19208] to FUBP1/FBP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FUBP1/FBP aa 150-250. The exact sequence is proprietary.
    Database link: Q96AE4

  • Positive control

    • WB: Jurkat, HeLa, Raji, C6, PC-12, NIH/3T3 and RAW 264.7 whole cell lysates; rat and mouse brain lysates. IHC-P: Human oligodendroglioma and cerebral cortex tissues; mouse cerebral cortex tissue; rat stomach tissue. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.
  • General notes

     

     

    Previously labelled as FUBP1. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab213525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 68 kDa).
IHC-P 1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/500.
Flow Cyt 1/600.
IP 1/30.

Target

  • Function

    Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.
  • Sequence similarities

    Contains 4 KH domains.
  • Post-translational
    modifications

    Ubiquitinated. This targets the protein for proteasome-mediated degradation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA helicase V antibody
    • far upstream element (FUSE) binding protein 1 antibody
    • Far upstream element (FUSE) binding protein 4 antibody
    • Far upstream element binding protein 1 antibody
    • far upstream element binding protein antibody
    • Far upstream element-binding protein 1 antibody
    • FBP antibody
    • FUBP antibody
    • Fubp1 antibody
    • FUBP1_HUMAN antibody
    • Fubp4 antibody
    • FUSE binding protein 1 antibody
    • FUSE-binding protein 1 antibody
    • HDH V antibody
    see all

Images

  • All lanes : Anti-FUBP1/FBP antibody [EPR19208] (ab213525) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 68 kDa
    Observed band size: 74 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression pattern observed is consistent with the literature (PMID: 24963357).

  • Lanes 1-2 : Anti-FUBP1/FBP antibody [EPR19208] (ab213525) at 1/1000 dilution
    Lanes 3-6 : Anti-FUBP1/FBP antibody [EPR19208] (ab213525) at 1/5000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 5 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 68 kDa
    Observed band size: 74 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-3/5/6: 1 second; Lane 4: 3 seconds.

    The expression pattern observed is consistent with the literature (PMID: 24963357).

  • Immunohistochemical analysis of paraffin-embedded human oligodendroglioma tissue labeling FUBP1/FBP with ab213525 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on the vascular endothelium of human oligodendroglioma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling FUBP1/FBP with ab213525 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on the neurons of human cerebral cortex is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling FUBP1/FBP with ab213525 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on the neurons of mouse cerebral cortex is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling FUBP1/FBP with ab213525 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on the neurons of rat stomach is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling FUBP1/FBP with ab213525 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling FUBP1/FBP with ab213525 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on NIH/3T3 cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling FUBP1/FBP with ab213525 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • FUBP1/FBP was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab213525 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213525 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: HeLa whole cell lysate 10µg (Input).

    Lane 2: ab213525 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213525 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    The expression pattern observed is consistent with the literature (PMID: 24963357).

References

ab213525 has not yet been referenced specifically in any publications.

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