- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-FUBP3 antibody
See all FUBP3 primary antibodies -
DescriptionRabbit polyclonal to FUBP3
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IPmore details
-
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Chicken, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant
-
Immunogen
Synthetic peptide corresponding to a region between amino acids 522 - 572 of Human FUBP3 (NP_003925.1)
-
Positive control
- HeLa, 293T or NIH 3T3 whole cell lysate.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
Storage bufferPreservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8 -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab108222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 62 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. |
Target
-
FunctionMay interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression.
-
Tissue specificityDetected in a number of cell lines.
-
Sequence similaritiesContains 4 KH domains.
-
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationNucleus.
- Information by UniProt
-
Database links
- Entrez Gene: 8939 Human
- Entrez Gene: 320267 Mouse
- Entrez Gene: 362106 Rat
- Omim: 603536 Human
- SwissProt: Q96I24 Human
- Unigene: 673029 Human
- Unigene: 98751 Human
-
Alternative names
- Far upstream element (FUSE) binding protein 3 antibody
- Far upstream element binding protein 3 antibody
- Far upstream element-binding protein 3 antibody
see all
Images
-
Western blot - Anti-FUBP3 antibody (ab108222)All lanes : Anti-FUBP3 antibody (ab108222) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 62 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-FUBP3 antibody (ab108222)Detection of FUBP3 by western blot of immunoprecipitate.
ab108222 at 1 µg/ml staining FUBP3 in HeLa whole cell lysate immunoprecipitated using ab108222 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: chemiluminescence with an exposure time of 30 seconds.
References
ab108222 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"