Anti-FUBP3 antibody (ab108222)
Key features and details
- Rabbit polyclonal to FUBP3
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FUBP3 antibody
See all FUBP3 primary antibodies -
Description
Rabbit polyclonal to FUBP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Chicken, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant
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Immunogen
Synthetic peptide corresponding to a region between amino acids 522 - 572 of Human FUBP3 (NP_003925.1)
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Positive control
- HeLa, 293T or NIH 3T3 whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab108222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 62 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. |
Target
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Function
May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. -
Tissue specificity
Detected in a number of cell lines. -
Sequence similarities
Contains 4 KH domains. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8939 Human
- Entrez Gene: 320267 Mouse
- Entrez Gene: 362106 Rat
- Omim: 603536 Human
- SwissProt: Q96I24 Human
- Unigene: 673029 Human
- Unigene: 98751 Human
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Alternative names
- Far upstream element (FUSE) binding protein 3 antibody
- Far upstream element binding protein 3 antibody
- Far upstream element-binding protein 3 antibody
see all
Images
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Western blot - Anti-FUBP3 antibody (ab108222)All lanes : Anti-FUBP3 antibody (ab108222) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 62 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-FUBP3 antibody (ab108222)Detection of FUBP3 by western blot of immunoprecipitate.
ab108222 at 1 µg/ml staining FUBP3 in HeLa whole cell lysate immunoprecipitated using ab108222 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
References (1)
ab108222 has been referenced in 1 publication.
- Lampe S et al. Identification of the TXNIP IRES and characterization of the impact of regulatory IRES trans-acting factors. Biochim Biophys Acta 1861:147-157 (2018). PubMed: 29378331
