Anti-FUBP3 antibody (ab108222)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-FUBP3 antibody
See all FUBP3 primary antibodies -
Description
Rabbit polyclonal to FUBP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Chicken, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant -
Immunogen
Synthetic peptide corresponding to a region between amino acids 522 - 572 of Human FUBP3 (NP_003925.1)
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Positive control
- HeLa, 293T or NIH 3T3 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab108222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/5000. Predicted molecular weight: 62 kDa. | |
IP | Use at 2-5 µg/mg of lysate. |
Target
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Function
May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. -
Tissue specificity
Detected in a number of cell lines. -
Sequence similarities
Contains 4 KH domains. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8939 Human
- Entrez Gene: 320267 Mouse
- Entrez Gene: 362106 Rat
- Omim: 603536 Human
- SwissProt: Q96I24 Human
- Unigene: 673029 Human
- Unigene: 98751 Human
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Alternative names
- Far upstream element (FUSE) binding protein 3 antibody
- Far upstream element binding protein 3 antibody
- Far upstream element-binding protein 3 antibody
see all
Images
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All lanes : Anti-FUBP3 antibody (ab108222) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 62 kDa
Exposure time: 3 minutes -
Detection of FUBP3 by western blot of immunoprecipitate.
ab108222 at 1 µg/ml staining FUBP3 in HeLa whole cell lysate immunoprecipitated using ab108222 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: chemiluminescence with an exposure time of 30 seconds.
Datasheets and documents
References
This product has been referenced in:
- Lampe S et al. Identification of the TXNIP IRES and characterization of the impact of regulatory IRES trans-acting factors. Biochim Biophys Acta 1861:147-157 (2018). Read more (PubMed: 29378331) »