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  1. Link

    fubp3-antibody-ab108222.pdf

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Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription TFIIH
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Anti-FUBP3 antibody (ab108222)

  • Datasheet
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  • Western blot - Anti-FUBP3 antibody (ab108222)
  • Immunoprecipitation - Anti-FUBP3 antibody (ab108222)

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  • Datasheet
  • References (1)
  • Protocols

Overview

  • Product name

    Anti-FUBP3 antibody
    See all FUBP3 primary antibodies
  • Description

    Rabbit polyclonal to FUBP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between amino acids 522 - 572 of Human FUBP3 (NP_003925.1)

  • Positive control

    • HeLa, 293T or NIH 3T3 whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • TFIIH
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • NIH 3T3 nuclear extract lysate (ab14874)
    • HeLa whole cell lysate (ab29545)
    • NIH 3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human FUBP3 protein (ab160271)

Applications

Our Abpromise guarantee covers the use of ab108222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 62 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression.
  • Tissue specificity

    Detected in a number of cell lines.
  • Sequence similarities

    Contains 4 KH domains.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q96I24 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8939 Human
    • Entrez Gene: 320267 Mouse
    • Entrez Gene: 362106 Rat
    • Omim: 603536 Human
    • SwissProt: Q96I24 Human
    • Unigene: 673029 Human
    • Unigene: 98751 Human
    • Alternative names

      • Far upstream element (FUSE) binding protein 3 antibody
      • Far upstream element binding protein 3 antibody
      • Far upstream element-binding protein 3 antibody
      • FBP3 antibody
      • FLJ25229 antibody
      • FUBP 3 antibody
      • FUBP3 antibody
      • FUBP3_HUMAN antibody
      • FUSE binding protein 3 antibody
      • FUSE-binding protein 3 antibody
      • OTTHUMP00000022369 antibody
      see all

    Images

    • Western blot - Anti-FUBP3 antibody (ab108222)
      Western blot - Anti-FUBP3 antibody (ab108222)
      All lanes : Anti-FUBP3 antibody (ab108222) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH 3T3 whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 62 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-FUBP3 antibody (ab108222)
      Immunoprecipitation - Anti-FUBP3 antibody (ab108222)
      Detection of FUBP3 by western blot of immunoprecipitate.
      ab108222 at 1 µg/ml staining FUBP3 in HeLa whole cell lysate immunoprecipitated using ab108222 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: chemiluminescence with an exposure time of 30 seconds.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Datasheets and documents

    • Datasheet
  • References

    This product has been referenced in:

    • Lampe S  et al. Identification of the TXNIP IRES and characterization of the impact of regulatory IRES trans-acting factors. Biochim Biophys Acta 1861:147-157 (2018). Read more (PubMed: 29378331) »
    See 1 Publication for this product

    Publishing research using ab108222? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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