Anti-FUBP3 antibody (ab108222)

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Overview

  • Product name

    Anti-FUBP3 antibody
    See all FUBP3 primary antibodies

  • Description

    Rabbit polyclonal to FUBP3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant

  • Immunogen

    Synthetic peptide corresponding to a region between amino acids 522 - 572 of Human FUBP3 (NP_003925.1)

  • Positive control

    • HeLa, 293T or NIH 3T3 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab108222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 62 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression.

  • Tissue specificity

    Detected in a number of cell lines.

  • Sequence similarities

    Contains 4 KH domains.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Far upstream element (FUSE) binding protein 3 antibody
      • Far upstream element binding protein 3 antibody
      • Far upstream element-binding protein 3 antibody
      • FBP3 antibody
      • FLJ25229 antibody
      • FUBP 3 antibody
      • FUBP3 antibody
      • FUBP3_HUMAN antibody
      • FUSE binding protein 3 antibody
      • FUSE-binding protein 3 antibody
      • OTTHUMP00000022369 antibody
      see all

    Images

    • Western blot - Anti-FUBP3 antibody (ab108222)
      Western blot - Anti-FUBP3 antibody (ab108222)
      All lanes : Anti-FUBP3 antibody (ab108222) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH 3T3 whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 62 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-FUBP3 antibody (ab108222)
      Immunoprecipitation - Anti-FUBP3 antibody (ab108222)
      Detection of FUBP3 by western blot of immunoprecipitate.
      ab108222 at 1 µg/ml staining FUBP3 in HeLa whole cell lysate immunoprecipitated using ab108222 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: chemiluminescence with an exposure time of 30 seconds.

    References

    This product has been referenced in:

    • Lampe S  et al. Identification of the TXNIP IRES and characterization of the impact of regulatory IRES trans-acting factors. Biochim Biophys Acta 1861:147-157 (2018). Read more (PubMed: 29378331) »
    See 1 Publication for this product

    Publishing research using ab108222? Please let us know so that we can cite the reference in this datasheet.

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