Overview

  • Product name

    Anti-FUBP3 antibody [EPR13174]
    See all FUBP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR13174] to FUBP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human FUBP3 aa 400-550. The exact sequence is proprietary.
    Database link: Q96I24

  • Positive control

    • HeLa, HepG2 and MCF-7 cell lysate. Human liver and placenta tissue. HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
IP 1/50.
ICC/IF 1/50 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression.
  • Tissue specificity

    Detected in a number of cell lines.
  • Sequence similarities

    Contains 4 KH domains.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Far upstream element (FUSE) binding protein 3 antibody
    • Far upstream element binding protein 3 antibody
    • Far upstream element-binding protein 3 antibody
    • FBP3 antibody
    • FLJ25229 antibody
    • FUBP 3 antibody
    • FUBP3 antibody
    • FUBP3_HUMAN antibody
    • FUSE binding protein 3 antibody
    • FUSE-binding protein 3 antibody
    • OTTHUMP00000022369 antibody
    see all

Images

  • All lanes : Anti-FUBP3 antibody [EPR13174] (ab181025) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : MCF-7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 62 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemical analysis of paraffin embedded Human liver tissue sections labeling FUBP3 using ab181025 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

  • Immunohistochemical analysis of paraffin embedded Human placenta tissue sections labeling FUBP3 using ab181025 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

  • Immunofluorescent analysis of acetone fixed HeLa cells labeling FUBP3 using ab181025 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

  • Lysate from HeLa cells was immunoprecipitated with ab181025 at a 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST

References

ab181025 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181025.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up