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  1. Link

    fuca1-antibody-epr12498-ab181357.pdf

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Signal Transduction Metabolism Amino Acids
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Recombinant
RabMAb

Recombinant Anti-FUCA1 antibody [EPR12498] (ab181357)

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  • Flow Cytometry - Anti-FUCA1 antibody [EPR12498] (ab181357)
  • Western blot - Anti-FUCA1 antibody [EPR12498] (ab181357)

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-FUCA1 antibody [EPR12498]
    See all FUCA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12498] to FUCA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human FUCA1 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P04066
    (Peptide available as ab208990)

  • Positive control

    • K562, Human fetal liver, and Human fetal kidney lysates, permeabilized K562 cells
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12498
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Anti-FUCA1 antibody [EPR12498] - BSA and Azide free (ab250485)
  • Immunizing Peptide (Blocking)

    • FUCA1 peptide (ab208990)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • K562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human FUCA1 protein (ab207120)

Applications

Our Abpromise guarantee covers the use of ab181357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.Can be blocked with FUCA1 peptide (ab208990).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.
    • Involvement in disease

      Defects in FUCA1 are the cause of fucosidosis (FUCA1D) [MIM:230000]. FUCA1D is an autosomal recessive lysosomal storage disease characterized by accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Clinical signs include facial dysmorphism, dysostosis multiplex, moderate hepatomegaly, severe intellectual deficit, deafness, and according to age, angiokeratomas.
    • Sequence similarities

      Belongs to the glycosyl hydrolase 29 family.
    • Cellular localization

      Lysosome.
    • Target information above from: UniProt accession P04066 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2517 Human
      • Entrez Gene: 71665 Mouse
      • Omim: 612280 Human
      • SwissProt: P04066 Human
      • SwissProt: Q99LJ1 Mouse
      • Unigene: 370858 Human
      • Unigene: 439940 Mouse
      • Alternative names

        • Alpha L fucosidase I antibody
        • Alpha L fucoside fucohydrolase antibody
        • Alpha-L-fucosidase 1 antibody
        • Alpha-L-fucosidase I antibody
        • Alpha-L-fucoside fucohydrolase 1 antibody
        • FUCA antibody
        • FUCA1 antibody
        • FUCO_HUMAN antibody
        • fucosidase, alpha-L, tissue antibody
        • fucosidase, alpha-L- 1, tissue antibody
        • Tissue alpha L fucosidase antibody
        • Tissue alpha-L-fucosidase antibody
        see all

      Images

      • Flow Cytometry - Anti-FUCA1 antibody [EPR12498] (ab181357)
        Flow Cytometry - Anti-FUCA1 antibody [EPR12498] (ab181357)

        Flow cytometric analysis of permeabilized K562 cells using ab181357 (red) at 1/10 dilution or a rabbit IgG (negative) (green).

      • Western blot - Anti-FUCA1 antibody [EPR12498] (ab181357)
        Western blot - Anti-FUCA1 antibody [EPR12498] (ab181357)
        All lanes : Anti-FUCA1 antibody [EPR12498] (ab181357) at 1/1000 dilution

        Lane 1 : K562 cell lysates
        Lane 2 : Human fetal liver lysates
        Lane 3 : Human fetal kidney lysates

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 54 kDa

      Protocols

      • Western blot protocols
      • Flow cytometry protocols

      Datasheets and documents

      • Datasheet
    • References

      ab181357 has not yet been referenced specifically in any publications.

      Publishing research using ab181357? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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