Recombinant Anti-FUCA1 antibody [EPR12498] (ab181357)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-FUCA1 antibody [EPR12498]
See all FUCA1 primary antibodies -
Description
Rabbit monoclonal [EPR12498] to FUCA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
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Positive control
- K562, Human fetal liver, and Human fetal kidney lysates, permeabilized K562 cells
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12498 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab181357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.Can be blocked with FUCA1 peptide (ab208990). | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. -
Involvement in disease
Defects in FUCA1 are the cause of fucosidosis (FUCA1D) [MIM:230000]. FUCA1D is an autosomal recessive lysosomal storage disease characterized by accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Clinical signs include facial dysmorphism, dysostosis multiplex, moderate hepatomegaly, severe intellectual deficit, deafness, and according to age, angiokeratomas. -
Sequence similarities
Belongs to the glycosyl hydrolase 29 family. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 2517 Human
- Entrez Gene: 71665 Mouse
- Omim: 612280 Human
- SwissProt: P04066 Human
- SwissProt: Q99LJ1 Mouse
- Unigene: 370858 Human
- Unigene: 439940 Mouse
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Alternative names
- Alpha L fucosidase I antibody
- Alpha L fucoside fucohydrolase antibody
- Alpha-L-fucosidase 1 antibody
see all
Images
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![Flow Cytometry - Anti-FUCA1 antibody [EPR12498] (ab181357)](https://www.abcam.com/ps/products/181/ab181357/Images/ab181357-210212-anti-fuca1-antibody-epr12498-flow-cytometry.jpg)
Flow Cytometry - Anti-FUCA1 antibody [EPR12498] (ab181357)Flow cytometric analysis of permeabilized K562 cells using ab181357 (red) at 1/10 dilution or a rabbit IgG (negative) (green).
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![Western blot - Anti-FUCA1 antibody [EPR12498] (ab181357)](https://www.abcam.com/ps/products/181/ab181357/Images/ab181357-210211-anti-fuca1-antibody-epr12498-western-blot.jpg)
Western blot - Anti-FUCA1 antibody [EPR12498] (ab181357)All lanes : Anti-FUCA1 antibody [EPR12498] (ab181357) at 1/1000 dilution
Lane 1 : K562 cell lysates
Lane 2 : Human fetal liver lysates
Lane 3 : Human fetal kidney lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa
Datasheets and documents
References
ab181357 has not yet been referenced specifically in any publications.
