Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12498] to FUCA1
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
Product nameAnti-FUCA1 antibody [EPR12498]
See all FUCA1 primary antibodies
DescriptionRabbit monoclonal [EPR12498] to FUCA1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Human
- K562, Human fetal liver, and Human fetal kidney lysates, permeabilized K562 cells
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 54 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionAlpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.
Involvement in diseaseDefects in FUCA1 are the cause of fucosidosis (FUCA1D) [MIM:230000]. FUCA1D is an autosomal recessive lysosomal storage disease characterized by accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Clinical signs include facial dysmorphism, dysostosis multiplex, moderate hepatomegaly, severe intellectual deficit, deafness, and according to age, angiokeratomas.
Sequence similaritiesBelongs to the glycosyl hydrolase 29 family.
- Information by UniProt
- Alpha L fucosidase I antibody
- Alpha L fucoside fucohydrolase antibody
- Alpha-L-fucosidase 1 antibody
Flow cytometric analysis of permeabilized K562 cells using ab181357 (red) at 1/10 dilution or a rabbit IgG (negative) (green).
All lanes : Anti-FUCA1 antibody [EPR12498] (ab181357) at 1/1000 dilution
Lane 1 : K562 cell lysates
Lane 2 : Human fetal liver lysates
Lane 3 : Human fetal kidney lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa
ab181357 has not yet been referenced specifically in any publications.