Overview

  • Product name

    Fumarase Activity Assay Kit (Colorimetric)
    See all Fumarase kits
  • Detection method

    Colorimetric
  • Sample type

    Tissue, Adherent cells, Suspension cells
  • Assay type

    Enzyme activity
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Fumarase Activity Assay kit (ab196992) provides a quick and easy method for monitoring fumarase activity in various samples. In this kit, fumarase converts fumarate into malate, which then reacts with Enzyme Mix to form an intermediate. The intermediate subsequently reduces the Developer to a colored product with strong absorbance at 450 nm.

    The assay is simple and high-throughput ready and can detect less than 50 U/ml of fumarase activity in variety of sample types.

  • Platform

    Microplate reader

Properties

Images

  • NADH Standard Curve.

  • Fumarase activity in mitochondria isolated from different sources.

  • Relative Fumarase activity was calculated in mitochondria prepared from bovine heart (13.75 μg), mouse liver (17.62 μg), yeast (S. cerevisiae) (37.8 μg), and MCF-7 cells (16.8 μg).

Protocols

References

ab196992 has not yet been referenced specifically in any publications.

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