Overview

  • Product name

    Fumarase Specific Activity Assay Kit
    See all Fumarase kits
  • Detection method

    Colorimetric
  • Assay type

    Enzyme activity
  • Species reactivity

    Reacts with: Rat, Cow, Human
    Does not react with: Mouse
  • Product overview

    ab110043 (MS780) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate fumarase (FH), which removes all other enzymes of the Krebs cycle and NAD+/NADH utilizing enzymes. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated FH is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.

  • Notes

    ab110043 (MS780) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate fumarase (FH), which removes all other enzymes of the Krebs cycle and NAD+/NADH utilizing enzymes. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated FH is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.

  • Platform

    Microplate reader

Properties

Images

  • Example data obtained using the Fumarase Specific Activity Assay Kit (ab110043).
  • Principle of a Sandwich ELISA.

Protocols

References

This product has been referenced in:

  • Perrier-Trudova V  et al. Fumarate Hydratase-deficient Cell Line NCCFH1 as a New In Vitro Model of Hereditary Papillary Renal Cell Carcinoma Type 2. Anticancer Res 35:6639-53 (2015). Read more (PubMed: 26637880) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us.

I can confirm that ab110043 Fumarase Specific Activity Assay Kit does worki with Rat, Cow and Human samples. This product will not work with mouse.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for contacting us.

The antibody used in this kit detects only human, rat and cow FH. It does not detect it in mouse samples. Therefore, we do not expect to see the activity from mouse samples in this immunocapture based activity assay.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

I am still waiting for mouse reactivity data for the fumarase assay, ab110043. I did confirm, however, that the ATP synthase assay, ab109714, does come with 12 strips of 8 wells each, and not a standard solid microplate.

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Question
Answer

Thank you very much for your interest in our products.

I just sent you Proforma Invoice (quotation) #45550 for the 4 kits. This quote will be good until Sept 30th when the discount expires. When you're ready to place the order, just call us and mention this number. If you need more reagents for your studies, you can use the discount code 4FOR3-TB3ME through Sept 30th to get the 4 for the price of 3 promotion on all products.

We would be very grateful if you could submit an Abreview aboutthese productsvia the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. You would have to do this manually, by working with our Scientific Support team through mailto:us.technical@abcam.com.

To find out more about our Abreview system, please see the following webpage: https://www.abcam.com/abreviews

And Abpoints: https://www.abcam.com/index.html?pageconfig=loyalty_rewards_list

We hope to receive your Abreviews. Thank you for your time and effort.

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