Key features and details
- Rabbit polyclonal to FUNDC1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FUNDC1 antibody
See all FUNDC1 primary antibodies
DescriptionRabbit polyclonal to FUNDC1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
- WB: Mouse heart lysate. IHC-P: Human glioma cancer tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab224722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionActs as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality control.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the FUN14 family.
DomainThe YXXL motif mediates the interaction with MAP1 LC3 family proteins MAP1LC3A, MAP1LC3B and GABARAP (PubMed:22267086).
modificationsPhosphorylation at Tyr-18 by SRC inhibits activation of mitophagy. Following hypoxia, dephosphorylated at Tyr-18, leading to interaction with MAP1 LC3 family proteins and triggering mitophagy.
Cellular localizationMitochondrion outer membrane.
- Information by UniProt
- FUN14 domain containing 1 antibody
- FUN14 domain containing protein 1 antibody
- FUN14 domain-containing protein 1 antibody
Anti-FUNDC1 antibody (ab224722) at 1/500 dilution + Mouse heart lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
Paraffin-embedded human glioma cancer tissue stained for FUNDC1 using ab224722 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224722 has been referenced in 3 publications.
- Wang J et al. Fundc1-dependent mitophagy is obligatory to ischemic preconditioning-conferred renoprotection in ischemic AKI via suppression of Drp1-mediated mitochondrial fission. Redox Biol 30:101415 (2020). PubMed: 31901590
- Wang ZC et al. MicroRNA-137 inhibits autophagy and chemosensitizes pancreatic cancer cells by targeting ATG5. Int J Biochem Cell Biol N/A:N/A (2019). PubMed: 30710750
- Yu W et al. Mst1 promotes cardiac ischemia-reperfusion injury by inhibiting the ERK-CREB pathway and repressing FUNDC1-mediated mitophagy. J Physiol Sci N/A:N/A (2018). PubMed: 29961191