Fura-2 Calcium Flux Assay Kit - No Wash, Ratiometric (ab176766)

Overview

  • Product name
    Fura-2 Calcium Flux Assay Kit - No Wash, Ratiometric
    See all Calcium kits
  • Detection method
    Fluorescent
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human, Chinese hamster
    Predicted to work with: Mammals
  • Product overview

    Fura-2 Calcium Assay Kit - No Wash (ab176766) is a ratiometric calcium assay that allows homogeneous measurement of intracellular calcium mobilization caused by activation of G-protein-coupled receptors (GPCR) or calcium channels. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with Fura-2 AM which can cross cell membrane. Once inside the cell, the lipophilic blocking groups of Fura-2 AM are cleaved by esterases, resulting in a negatively charged fluorescent dye that stays inside cells and its fluorescence wavelength is blue-shifted upon binding to calcium. When cells are stimulated with agonists, the receptor signals the release of intracellular calcium, which greatly increase the fluorescence intensity of Fura-2 at the short wavelength.


    The characteristics of its ratio measurements reduce the effects of uneven dye loading and cell numbers, dye leakage and photo bleaching. The 340/380 nm excitation ratio allows accurate measurements of the intracellular Ca2+ concentration and makes this kit an ideal tool for more accurate measurement of cellular calcium concentration compared to Fluo-8 of the single wavelength.


    ab176766 provides an optimized assay method with no washing step for monitoring the G-protein-coupled receptors and calcium channels using HTS instrumentation. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.

  • Notes

    This product is intended to be used for monitoring calcium fluctuations in vivo in live cells using the following HTS imaging plate readers: FLIPR™, FDSS, BMG NOVOstar™, FLexStation, ViewLux, IN Cell Analyzer or Arrayscan.

  • Platform
    Microplate

Properties

Associated products

Images

  • CHO-K1 cells were seeded overnight at 40,000 cells/100 μL/well in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 μL of the Fura-2 No Wash Calcium Assay Kit (ab176766) for 1 hour at room temperature. ATP (50 μL/well) was added to achieve the final indicated concentrations.

Protocols

References

ab176766 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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