Key features and details
- Goat polyclonal to Furin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Furin antibody
See all Furin primary antibodies
DescriptionGoat polyclonal to Furin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- HEK293 lysate overexpressing Human Furin with C-terminal MYC tag.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab177231 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Our Abpromise guarantee covers the use of ab177231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 87 kDa.
A 1 hour primary incubation is recommended for this product.
FunctionFurin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.
Tissue specificitySeems to be expressed ubiquitously.
Sequence similaritiesBelongs to the peptidase S8 family. Furin subfamily.
Contains 1 homo B/P domain.
DomainContains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.
modificationsThe inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms.
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
- Information by UniProt
- Dibasic processing enzyme antibody
- Dibasic-processing enzyme antibody
- FES upstream region antibody
Lanes 1-2 : Anti-Furin antibody (ab177231) at 1 µg/ml
Lane 3 : anti-MYC tag at 1/1000 dilution
Lanes 1 & 3 : HEK293 lysate (in RIPA buffer) overexpressing Human Furin with C-terminal MYC tag
Lane 2 : Mock-transfected HEK293 lysate (in RIPA buffer)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 87 kDa
Primary incubations were for 1 hour.
ab177231 has not yet been referenced specifically in any publications.