- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Furin antibody
See all Furin primary antibodies -
DescriptionGoat polyclonal to Furin
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Host speciesGoat
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Synthetic peptide corresponding to Human Furin aa 104-116 (internal sequence) (Cysteine residue).
Sequence:RTKRDVYQEPTDP
Database link: NP_002560.1 -
Positive control
- HEK293 lysate overexpressing Human Furin with C-terminal MYC tag.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab177231 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Related Products
Applications
Our Abpromise guarantee covers the use of ab177231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 87 kDa. |
Target
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FunctionFurin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.
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Tissue specificitySeems to be expressed ubiquitously.
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Sequence similaritiesBelongs to the peptidase S8 family. Furin subfamily.
Contains 1 homo B/P domain. -
DomainContains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.
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Post-translational
modificationsThe inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms. -
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
- Information by UniProt
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Database links
- Entrez Gene: 281374 Cow
- Entrez Gene: 5045 Human
- Entrez Gene: 18550 Mouse
- Entrez Gene: 54281 Rat
- Omim: 136950 Human
- SwissProt: Q28193 Cow
- SwissProt: P09958 Human
- SwissProt: P23188 Mouse
see all -
Alternative names
- Dibasic processing enzyme antibody
- Dibasic-processing enzyme antibody
- FES upstream region antibody
see all
Images
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Western blot - Anti-Furin antibody (ab177231)Lanes 1-2 : Anti-Furin antibody (ab177231) at 1 µg/ml
Lane 3 : anti-MYC tag at 1/1000 dilution
Lanes 1 & 3 : HEK293 lysate (in RIPA buffer) overexpressing Human Furin with C-terminal MYC tag
Lane 2 : Mock-transfected HEK293 lysate (in RIPA buffer)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 87 kDaPrimary incubations were for 1 hour.
Protocols
References
ab177231 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"