Anti-Furin antibody (ab177231)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-Furin antibody
See all Furin primary antibodies -
Description
Goat polyclonal to Furin -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
-
Immunogen
Synthetic peptide corresponding to Human Furin aa 104-116 (internal sequence) (Cysteine residue).
Sequence:RTKRDVYQEPTDP
Database link: NP_002560.1 -
Positive control
- HEK293 lysate overexpressing Human Furin with C-terminal MYC tag.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab177231 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab177231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 87 kDa. |
Target
-
Function
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. -
Tissue specificity
Seems to be expressed ubiquitously. -
Sequence similarities
Belongs to the peptidase S8 family. Furin subfamily.
Contains 1 homo B/P domain. -
Domain
Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface. -
Post-translational
modificationsThe inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin. - Information by UniProt
-
Database links
- Entrez Gene: 281374 Cow
- Entrez Gene: 5045 Human
- Entrez Gene: 18550 Mouse
- Entrez Gene: 54281 Rat
- Omim: 136950 Human
- SwissProt: Q28193 Cow
- SwissProt: P09958 Human
- SwissProt: P23188 Mouse
see all -
Alternative names
- Dibasic processing enzyme antibody
- Dibasic-processing enzyme antibody
- FES upstream region antibody
see all
Images
-
Western blot - Anti-Furin antibody (ab177231)Lanes 1-2 : Anti-Furin antibody (ab177231) at 1 µg/ml
Lane 3 : anti-MYC tag at 1/1000 dilution
Lanes 1 & 3 : HEK293 lysate (in RIPA buffer) overexpressing Human Furin with C-terminal MYC tag
Lane 2 : Mock-transfected HEK293 lysate (in RIPA buffer)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 87 kDaPrimary incubations were for 1 hour.
Protocols
Datasheets and documents
References
ab177231 has not yet been referenced specifically in any publications.
