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So, you are saying, the 60kD band is the Signal Sequence (˜24AA)+ Propeptide (˜130AA) + catalytic domain (˜280)+ partial RGD domain.
Asked on May 18 2012
Sorry for this misunderstanding.
We believe that the antibody recognizes the mature form of the protein. The pro-peptide is expected at 87kDa and once the signal and pro-peptide are cleaved off the protein, it migrates at 63 kDa.
I hope this is helpful and wish you a good weekend.
Answered on May 18 2012