Key features and details
- Rabbit polyclonal to Furin - Aminoterminal end
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Furin antibody - Aminoterminal end
See all Furin primary antibodies
DescriptionRabbit polyclonal to Furin - Aminoterminal end
Specificityab28547 recognizes subtilisin-like serine protease furin.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Furin (N terminal).
(Peptide available as
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 2.9% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody has been peptide-affinity purified.
Our Abpromise guarantee covers the use of ab28547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 88 kDa.
1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Glycosylation and other post-translational modifications make the pre-pro furin run at 110-104 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa. Dilution optimised using Chromogenic detection.
FunctionFurin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.
Tissue specificitySeems to be expressed ubiquitously.
Sequence similaritiesBelongs to the peptidase S8 family. Furin subfamily.
Contains 1 homo B/P domain.
DomainContains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.
modificationsThe inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms.
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
- Information by UniProt
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ab28547 has been referenced in 6 publications.
- Braun E et al. Guanylate-Binding Proteins 2 and 5 Exert Broad Antiviral Activity by Inhibiting Furin-Mediated Processing of Viral Envelope Proteins. Cell Rep 27:2092-2104.e10 (2019). PubMed: 31091448
- Jaaks P et al. The proprotein convertase furin is required to maintain viability of alveolar rhabdomyosarcoma cells. Oncotarget 7:76743-76755 (2016). Human . PubMed: 27572312
- Jaaks P et al. The Proprotein Convertase Furin Contributes to Rhabdomyosarcoma Malignancy by Promoting Vascularization, Migration and Invasion. PLoS One 11:e0161396 (2016). IHC . PubMed: 27548722
- Sathyamurthy M et al. Characterization and expression of proprotein convertases in CHO cells: Efficient proteolytic maturation of human bone morphogenetic protein-7. Biotechnol Bioeng N/A:N/A (2014). WB . PubMed: 25219685
- Cekic M et al. Progesterone treatment alters neurotrophin/proneurotrophin balance and receptor expression in rats with traumatic brain injury. Restor Neurol Neurosci 30:115-26 (2012). WB ; Rat . PubMed: 22232032
- Hajdin K et al. Furin targeted drug delivery for treatment of rhabdomyosarcoma in a mouse model. PLoS One 5:e10445 (2010). IHC-Fr ; Human . PubMed: 20454619