• Product name
    Anti-Furin antibody - Aminoterminal end
    See all Furin primary antibodies
  • Description
    Rabbit polyclonal to Furin - Aminoterminal end
  • Host species
  • Specificity
    ab28547 recognizes subtilisin-like serine protease furin.
  • Tested applications
    Suitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Furin (N terminal).
    (Peptide available as ab41397)

  • Positive control
    • Furin is ubiquitously expressed at low levels, but found in highest concentration in the liver and kidneys, lower levels in brain, spleen, thymus, and smaller levels in most other tissues. Endogenous furin was detected in lysates from LNCaP and DU145 prostate carcinoma cell lines.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.4
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 2.9% Sodium chloride, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody has been peptide-affinity purified.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab28547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 88 kDa.

1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Glycosylation and other post-translational modifications make the pre-pro furin run at 110-104 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa. Dilution optimised using Chromogenic detection.

IHC-Fr Use at an assay dependent concentration. PubMed: 20454619


  • Function
    Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.
  • Tissue specificity
    Seems to be expressed ubiquitously.
  • Sequence similarities
    Belongs to the peptidase S8 family. Furin subfamily.
    Contains 1 homo B/P domain.
  • Domain
    Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.
  • Post-translational
    The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
    Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms.
  • Cellular localization
    Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dibasic processing enzyme antibody
    • Dibasic-processing enzyme antibody
    • FES upstream region antibody
    • FUR antibody
    • FURIN antibody
    • Furin membrane associated receptor protein antibody
    • FURIN_HUMAN antibody
    • PACE antibody
    • Paired basic amino acid residue cleaving enzyme antibody
    • Paired basic amino acid residue-cleaving enzyme antibody
    • PCSK3 antibody
    • Proprotein convertase subtilisin/kexin type 3 antibody
    • SPC1 antibody
    see all


  • All lanes : Anti-Furin antibody - Aminoterminal end (ab28547) at 1/1000 dilution

    Lane 1 : Furin at 0.05 µg
    Lane 2 : Furin at 0.01 µg
    Lane 3 : Furin at 0.001 µg

    Predicted band size: 88 kDa
    Observed band size: 88 kDa

  • ab28547 staining Furin (red) in mouse endothelial tissue by Immunohistochemistry (Frozen sections).
    Mice were perfused, tumor and control organs were dissected and frozen in O.C.T. embedding medium. 10 µm cryosections were washed with PBS, fixed for 15 minutes in ice-cold methanol and air-dried. Primary antibody was used at a dilution of 1/1500. The secondary antibody was an Alexa Fluor 594-labeled IgG at a 1/300 dilution. Nuclei are visualized by DAPI staining (blue).


This product has been referenced in:
  • Jaaks P  et al. The proprotein convertase furin is required to maintain viability of alveolar rhabdomyosarcoma cells. Oncotarget 7:76743-76755 (2016). Human . Read more (PubMed: 27572312) »
  • Jaaks P  et al. The Proprotein Convertase Furin Contributes to Rhabdomyosarcoma Malignancy by Promoting Vascularization, Migration and Invasion. PLoS One 11:e0161396 (2016). IHC . Read more (PubMed: 27548722) »
See all 5 Publications for this product

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