RecombinantRabMAb

Recombinant Anti-Furin antibody [EPR14674] (ab183495)

Reviews (2) Submit a question References (4)
Western blot - Anti-Furin antibody [EPR14674] (ab183495)
  • Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)
  • Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)
  • Anti-Furin antibody [EPR14674] (ab183495)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14674] to Furin
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Furin antibody [EPR14674]
    See all Furin primary antibodies

  • Description

    Rabbit monoclonal [EPR14674] to Furin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Furin aa 200-350. The exact sequence is proprietary.
    Database link: P09958

  • Positive control

    • HepG2, Hela, Caco-2 and U87-MG lysates, Human gastric carcinoma, Human prostate tissues, Hela and HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 20% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14674

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).

Target

  • Function

    Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.

  • Tissue specificity

    Seems to be expressed ubiquitously.

  • Sequence similarities

    Belongs to the peptidase S8 family. Furin subfamily.
    Contains 1 homo B/P domain.

  • Domain

    Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.

  • Post-translational
    modifications

    The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
    Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms.

  • Cellular localization

    Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Dibasic processing enzyme antibody
    • Dibasic-processing enzyme antibody
    • FES upstream region antibody
    • FUR antibody
    • FURIN antibody
    • Furin membrane associated receptor protein antibody
    • FURIN_HUMAN antibody
    • PACE antibody
    • Paired basic amino acid residue cleaving enzyme antibody
    • Paired basic amino acid residue-cleaving enzyme antibody
    • PCSK3 antibody
    • Proprotein convertase subtilisin/kexin type 3 antibody
    • SPC1 antibody
    see all

Images

  • Western blot - Anti-Furin antibody [EPR14674] (ab183495)
    Western blot - Anti-Furin antibody [EPR14674] (ab183495)
    All lanes : Anti-Furin antibody [EPR14674] (ab183495) at 1/5000 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : U87-MG whole cell lysate
    Lane 4 : Caco-2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 87 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)
    Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)

    Immunocytochemical analysis of HepG2 cells fixed in 4% paraformaldehyde labeling ab183495 at 1/250 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200.  Counterstained with DAPI.

  • Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)
    Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)

    Immunocytochemical analysis of HeLa cells fixed in 20 °C acetone labeling Furin with ab183495 at 1/250 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 dilution.  Counterstained with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)

    Immunohistochemical analysis of paraffin-embedded Human prostate labeling Furin with ab183495 at 1/50 dilution and counterstained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)

    Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling Furin with ab183495 at 1/50 dilution and counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Furin antibody [EPR14674] (ab183495)
    Anti-Furin antibody [EPR14674] (ab183495)

References (4)

Publishing research using ab183495? Please let us know so that we can cite the reference in this datasheet.

ab183495 has been referenced in 4 publications.

  • Yang X  et al. FURIN Expression in Vascular Endothelial Cells Is Modulated by a Coronary Artery Disease-Associated Genetic Variant and Influences Monocyte Transendothelial Migration. J Am Heart Assoc 9:e014333 (2020). PubMed: 32067586
  • Wang CA  et al. DUSP2 regulates extracellular vesicle-VEGF-C secretion and pancreatic cancer early dissemination. J Extracell Vesicles 9:1746529 (2020). PubMed: 32341770
  • Yuan Y  et al. Furin-mediated intracellular self-assembly of olsalazine nanoparticles for enhanced magnetic resonance imaging and tumour therapy. Nat Mater 18:1376-1383 (2019). PubMed: 31636420
  • Yang Y  et al. Transgenic overexpression of furin increases epileptic susceptibility. Cell Death Dis 9:1058 (2018). PubMed: 30333479

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Furin antibody [EPR14674]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Purified protein (mouse neural stem cells)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
mouse neural stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Dr. Robert Kupp

Verified customer

Submitted Oct 20 2020

Immunoprecipitation abreview for Anti-Furin antibody [EPR14674]

 Excellent
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (pancreatic cancer cell)
Total protein in input
1.5e+006 cells
Immuno-precipitation step
Other - Dynabeads
Specification
pancreatic cancer cell
Read More

Abcam user community

Verified customer

Submitted Oct 20 2017

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