- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Furin antibody [EPR14674]
See all Furin primary antibodies -
DescriptionRabbit monoclonal [EPR14674] to Furin
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment within Human Furin aa 200-350. The exact sequence is proprietary.
Database link: P09958 -
Positive control
- HepG2, Hela, Caco-2 and U87-MG lysates, Human gastric carcinoma, Human prostate tissues, Hela and HepG2 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 20% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR14674
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Related Products
Applications
Our Abpromise guarantee covers the use of ab183495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/250. | |
| IHC-P | 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa). |
Target
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FunctionFurin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.
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Tissue specificitySeems to be expressed ubiquitously.
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Sequence similaritiesBelongs to the peptidase S8 family. Furin subfamily.
Contains 1 homo B/P domain. -
DomainContains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.
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Post-translational
modificationsThe inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms. -
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
- Information by UniProt
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Database links
- Entrez Gene: 5045 Human
- Entrez Gene: 18550 Mouse
- Entrez Gene: 54281 Rat
- Omim: 136950 Human
- SwissProt: P09958 Human
- SwissProt: P23188 Mouse
- SwissProt: P23377 Rat
- Unigene: 513153 Human
see all -
Alternative names
- Dibasic processing enzyme antibody
- Dibasic-processing enzyme antibody
- FES upstream region antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)](https://www.abcam.com/ps/products/183/ab183495/Images/ab183495-214859-icc1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)Immunocytochemical analysis of HepG2 cells fixed in 4% paraformaldehyde labeling ab183495 at 1/250 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200. Counterstained with DAPI.
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![Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)](https://www.abcam.com/ps/products/183/ab183495/Images/ab183495-214858-icc1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)Immunocytochemical analysis of HeLa cells fixed in 20 °C acetone labeling Furin with ab183495 at 1/250 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 dilution. Counterstained with DAPI.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)](https://www.abcam.com/ps/products/183/ab183495/Images/ab183495-214857-ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)Immunohistochemical analysis of paraffin-embedded Human prostate labeling Furin with ab183495 at 1/50 dilution and counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)](https://www.abcam.com/ps/products/183/ab183495/Images/ab183495-214856-ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling Furin with ab183495 at 1/50 dilution and counterstained with Hematoxylin.
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![Western blot - Anti-Furin antibody [EPR14674] (ab183495)](https://www.abcam.com/ps/products/183/ab183495/Images/ab183495-214855-WB1.jpg)
Western blot - Anti-Furin antibody [EPR14674] (ab183495)All lanes : Anti-Furin antibody [EPR14674] (ab183495) at 1/5000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : U87-MG whole cell lysate
Lane 4 : Caco-2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 87 kDa
Protocols
References
ab183495 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"