Recombinant
RabMAb

Recombinant Anti-Furin antibody [EPR14674] (ab183495)

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Overview

  • Product name

    Anti-Furin antibody [EPR14674]
    See all Furin primary antibodies

  • Description

    Rabbit monoclonal [EPR14674] to Furin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Furin aa 200-350. The exact sequence is proprietary.
    Database link: P09958

  • Positive control

    • HepG2, Hela, Caco-2 and U87-MG lysates, Human gastric carcinoma, Human prostate tissues, Hela and HepG2 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 20% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14674

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).

Target

  • Function

    Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.

  • Tissue specificity

    Seems to be expressed ubiquitously.

  • Sequence similarities

    Belongs to the peptidase S8 family. Furin subfamily.
    Contains 1 homo B/P domain.

  • Domain

    Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.

  • Post-translational
    modifications

    The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
    Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms.

  • Cellular localization

    Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Dibasic processing enzyme antibody
    • Dibasic-processing enzyme antibody
    • FES upstream region antibody
    • FUR antibody
    • FURIN antibody
    • Furin membrane associated receptor protein antibody
    • FURIN_HUMAN antibody
    • PACE antibody
    • Paired basic amino acid residue cleaving enzyme antibody
    • Paired basic amino acid residue-cleaving enzyme antibody
    • PCSK3 antibody
    • Proprotein convertase subtilisin/kexin type 3 antibody
    • SPC1 antibody
    see all

Images

  • Western blot - Anti-Furin antibody [EPR14674] (ab183495)
    Western blot - Anti-Furin antibody [EPR14674] (ab183495)
    All lanes : Anti-Furin antibody [EPR14674] (ab183495) at 1/5000 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : U87-MG whole cell lysate
    Lane 4 : Caco-2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 87 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)
    Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)

    Immunocytochemical analysis of HepG2 cells fixed in 4% paraformaldehyde labeling ab183495 at 1/250 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200.  Counterstained with DAPI.

  • Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)
    Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)

    Immunocytochemical analysis of HeLa cells fixed in 20 °C acetone labeling Furin with ab183495 at 1/250 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 dilution.  Counterstained with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)

    Immunohistochemical analysis of paraffin-embedded Human prostate labeling Furin with ab183495 at 1/50 dilution and counterstained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)

    Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling Furin with ab183495 at 1/50 dilution and counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

This product has been referenced in:

See 1 Publication for this product

Publishing research using ab183495? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Immunoprecipitation abreview for Anti-Furin antibody [EPR14674]

 Excellent
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (pancreatic cancer cell)
Total protein in input
1.5e+006 cells
Immuno-precipitation step
Other - Dynabeads
Specification
pancreatic cancer cell
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Submitted Oct 20 2017

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