Anti-Furin antibody [MM0298-4G31] (ab89558)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Furin antibody [MM0298-4G31]
See all Furin primary antibodies -
Description
Mouse monoclonal [MM0298-4G31] to Furin -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant Human Furin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and filtered through a 0.2 µm filter. -
Clonality
Monoclonal -
Clone number
MM0298-4G31 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab89558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| WB | 1/500 - 1/1000. Predicted molecular weight: 88 kDa. | |
| IHC-P | 1/20 - 1/100. |
Target
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Function
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. -
Tissue specificity
Seems to be expressed ubiquitously. -
Sequence similarities
Belongs to the peptidase S8 family. Furin subfamily.
Contains 1 homo B/P domain. -
Domain
Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface. -
Post-translational
modificationsThe inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin. - Information by UniProt
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Database links
- Entrez Gene: 5045 Human
- Omim: 136950 Human
- SwissProt: P09958 Human
- Unigene: 513153 Human
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Alternative names
- Dibasic processing enzyme antibody
- Dibasic-processing enzyme antibody
- FES upstream region antibody
see all
Images
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![Flow Cytometry - Anti-Furin antibody [MM0298-4G31] (ab89558)](https://www.abcam.com/ps/products/89/ab89558/Images/ab89558-105300-anti-furin-antibody-mm0298-4g31-flow-cytometry.jpg)
Flow Cytometry - Anti-Furin antibody [MM0298-4G31] (ab89558)Overlay histogram showing HeLa cells stained with ab89558 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab89558, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Datasheets and documents
References
ab89558 has not yet been referenced specifically in any publications.
