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Cell Biology Proteolysis / Ubiquitin Proteolytic enzymes Serine protease Proprotein Convertases
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Anti-Furin antibody [MM0298-4G31] (ab89558)

  • Datasheet
  • SDS
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Flow Cytometry - Anti-Furin antibody [MM0298-4G31] (ab89558)

    Key features and details

    • Mouse monoclonal [MM0298-4G31] to Furin
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Protein
    Recombinant Furin protein (ab6032)

    View more associated products

    Overview

    • Product name

      Anti-Furin antibody [MM0298-4G31]
      See all Furin primary antibodies
    • Description

      Mouse monoclonal [MM0298-4G31] to Furin
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      Flow Cyt
      Human
      See all applications and species data
    • Immunogen

      Recombinant Human Furin

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and filtered through a 0.2 µm filter.
    • Clonality

      Monoclonal
    • Clone number

      MM0298-4G31
    • Isotype

      IgG1
    • Research areas

      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Serine protease
      • Proprotein Convertases

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
      • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
      • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    • Recombinant Protein

      • Recombinant Furin protein (ab6032)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab89558 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    Flow Cyt
    Human
    Application Abreviews Notes
    Flow Cyt
    Use 2µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Notes
    Flow Cyt
    Use 2µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.
    • Tissue specificity

      Seems to be expressed ubiquitously.
    • Sequence similarities

      Belongs to the peptidase S8 family. Furin subfamily.
      Contains 1 homo B/P domain.
    • Domain

      Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.
    • Post-translational
      modifications

      The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
      Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms.
    • Cellular localization

      Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
    • Target information above from: UniProt accession P09958 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5045 Human
      • Omim: 136950 Human
      • SwissProt: P09958 Human
      • Unigene: 513153 Human
      • Alternative names

        • Dibasic processing enzyme antibody
        • Dibasic-processing enzyme antibody
        • FES upstream region antibody
        • FUR antibody
        • FURIN antibody
        • Furin membrane associated receptor protein antibody
        • FURIN_HUMAN antibody
        • PACE antibody
        • Paired basic amino acid residue cleaving enzyme antibody
        • Paired basic amino acid residue-cleaving enzyme antibody
        • PCSK3 antibody
        • Proprotein convertase subtilisin/kexin type 3 antibody
        • SPC1 antibody
        see all

      Images

      • Flow Cytometry - Anti-Furin antibody [MM0298-4G31] (ab89558)
        Flow Cytometry - Anti-Furin antibody [MM0298-4G31] (ab89558)
        Overlay histogram showing HeLa cells stained with ab89558 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab89558, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab89558? Please let us know so that we can cite the reference in this datasheet.

      ab89558 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Immunocytochemistry/ Immunofluorescence abreview for Anti-Furin antibody [MM0298-4G31]

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa)
      Specification
      HeLa
      Permeabilization
      Yes - 0.5% Triton X100 in PBS
      Fixative
      Paraformaldehyde
      Read More

      Dr. Kirk Mcmanus

      Verified customer

      Submitted Jan 28 2014

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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