Anti-FUT2 antibody (ab177239)
Key features and details
- Goat polyclonal to FUT2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FUT2 antibody -
Description
Goat polyclonal to FUT2 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla -
Immunogen
Synthetic peptide corresponding to Human FUT2 aa 27-40 (internal sequence) (Cysteine residue). (near N terminus).
Sequence:HVQQRLAKIQAMWE
Database link: NP_000502.4 -
Positive control
- Human liver lysate.
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General notes
Reported variants represent identical protein: NP_001091107.1, NP_000502.4.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab177239 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 39 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 39 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Creates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble A and B antigen synthesis pathway. H and Se enzymes fucosylate the same acceptor substrates but exhibit different Km values. -
Tissue specificity
Small intestine, colon and lung. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyltransferase 11 family. -
Cellular localization
Golgi apparatus > Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi. - Information by UniProt
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Database links
- Entrez Gene: 468944 Chimpanzee
- Entrez Gene: 101148312 Gorilla
- Entrez Gene: 2524 Human
- Omim: 182100 Human
- SwissProt: O77485 Chimpanzee
- SwissProt: O77486 Gorilla
- SwissProt: Q10981 Human
- Unigene: 579928 Human
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Alternative names
- 2)FT 2 antibody
- Alpha (1,2) fucosyltransferase antibody
- Alpha(1 antibody
see all
Images
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Anti-FUT2 antibody (ab177239) at 1 µg/ml + Human liver lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
Datasheets and documents
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Datasheet download
References (1)
ab177239 has been referenced in 1 publication.
- Lili Q et al. Clostridium butyricum Induces the Production and Glycosylation of Mucins in HT-29 Cells. Front Cell Infect Microbiol 11:668766 (2021). PubMed: 34222040