Anti-FUT3 antibody (ab110082)
Key features and details
- Rabbit polyclonal to FUT3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FUT3 antibody
See all FUT3 primary antibodies -
Description
Rabbit polyclonal to FUT3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
A synthesized peptide derived from an internal sequence of FUT3.
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Positive control
- Human colon carcinoma tissue; extracts from K562 cells and Jurkat cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab110082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 42 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links. -
Tissue specificity
Highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyltransferase 10 family. -
Cellular localization
Golgi apparatus > Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi. - Information by UniProt
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Database links
- Entrez Gene: 2525 Human
- Omim: 111100 Human
- SwissProt: P21217 Human
- Unigene: 169238 Human
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Alternative names
- Alpha-(1,3/1,4)-fucosyltransferase antibody
- Blood group Lewis alpha-4-fucosyltransferase antibody
- CD174 antibody
see all
Images
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Western blot - Anti-FUT3 antibody (ab110082)All lanes : Anti-FUT3 antibody (ab110082) at 1/500 dilution
Lane 1 : extracts from K562 cells
Lane 2 : extracts from Jurkat cells
Lane 3 : extracts from Jurkat cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUT3 antibody (ab110082)Immunohistochemical analysis of Blood Group Lewis b in paraffin embedded Human colon carcinoma tissue, using a 1/50 dilution of ab110082 with (right image) or without (left image) immunizig peptide.
Protocols
Datasheets and documents
References (3)
ab110082 has been referenced in 3 publications.
- Esposito M et al. Bone vascular niche E-selectin induces mesenchymal-epithelial transition and Wnt activation in cancer cells to promote bone metastasis. Nat Cell Biol N/A:N/A (2019). PubMed: 30988423
- Zhan L et al. Knockdown of FUT3 disrupts the proliferation, migration, tumorigenesis and TGF-ß induced EMT in pancreatic cancer cells. Oncol Lett 16:924-930 (2018). PubMed: 29963165
- Meng L et al. High expression of FUT3 is linked to poor prognosis in clear cell renal cell carcinoma. Oncotarget 8:61036-61047 (2017). PubMed: 28977844
