Anti-FUT3 antibody (ab110082)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-FUT3 antibody
See all FUT3 primary antibodies -
Description
Rabbit polyclonal to FUT3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
A synthesized peptide derived from an internal sequence of FUT3.
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Positive control
- Human colon carcinoma tissue; extracts from K562 cells and Jurkat cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.88% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab110082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 42 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links. -
Tissue specificity
Highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyltransferase 10 family. -
Cellular localization
Golgi apparatus > Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi. - Information by UniProt
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Database links
- Entrez Gene: 2525 Human
- Omim: 111100 Human
- SwissProt: P21217 Human
- Unigene: 169238 Human
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Alternative names
- Alpha-(1,3/1,4)-fucosyltransferase antibody
- Blood group Lewis alpha-4-fucosyltransferase antibody
- CD174 antibody
see all
Images
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Western blot - Anti-FUT3 antibody (ab110082)All lanes : Anti-FUT3 antibody (ab110082) at 1/500 dilution
Lane 1 : extracts from K562 cells
Lane 2 : extracts from Jurkat cells
Lane 3 : extracts from Jurkat cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUT3 antibody (ab110082)Immunohistochemical analysis of Blood Group Lewis b in paraffin embedded Human colon carcinoma tissue, using a 1/50 dilution of ab110082 with (right image) or without (left image) immunizig peptide.
Datasheets and documents
References
This product has been referenced in:
- Esposito M et al. Bone vascular niche E-selectin induces mesenchymal-epithelial transition and Wnt activation in cancer cells to promote bone metastasis. Nat Cell Biol N/A:N/A (2019). Read more (PubMed: 30988423) »
- Zhan L et al. Knockdown of FUT3 disrupts the proliferation, migration, tumorigenesis and TGF-ß induced EMT in pancreatic cancer cells. Oncol Lett 16:924-930 (2018). Read more (PubMed: 29963165) »
