Anti-FUT4 antibody (ab231561)
Key features and details
- Rabbit polyclonal to FUT4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FUT4 antibody
See all FUT4 primary antibodies -
Description
Rabbit polyclonal to FUT4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Mouse FUT4 aa 100-400. (Expressed in E.coli).
Database link: Q11127 -
Positive control
- IHC-P: Mouse lung, brain and kidney tissues. WB: Mouse stomach lysate; HepG2, HeLa and Jurkat cell lysates; Recombinant mouse FUT4 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231561 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 49 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 49 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyltransferase 10 family. -
Cellular localization
Golgi apparatus > Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi. - Information by UniProt
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Database links
- Entrez Gene: 10690 Human
- Entrez Gene: 2526 Human
- Entrez Gene: 2527 Human
- Entrez Gene: 2528 Human
- Entrez Gene: 2529 Human
- Entrez Gene: 14345 Mouse
- Entrez Gene: 14347 Mouse
- Entrez Gene: 14348 Mouse
see all -
Alternative names
- 3)-fucosyltransferase antibody
- Alpha-(1 antibody
- Alpha-(1,3)-fucosyltransferase antibody
see all
Images
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Paraffin-embedded mouse lung tissue stained for CD15 using ab231561 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded mouse kidney tissue stained for CD15 using ab231561 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded mouse stomach tissue stained for CD15 using ab231561 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded mouse brain tissue stained for CD15 using ab231561 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-FUT4 antibody (ab231561) at 2 µg/ml + Recombinant mouse CD15 protein
Predicted band size: 49 kDa -
Anti-FUT4 antibody (ab231561) at 2 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Predicted band size: 49 kDa -
Anti-FUT4 antibody (ab231561) at 2 µg/ml + Mouse stomach lysate
Predicted band size: 49 kDa -
Anti-FUT4 antibody (ab231561) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 49 kDa -
Anti-FUT4 antibody (ab231561) at 2 µg/ml + Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Predicted band size: 49 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231561 has not yet been referenced specifically in any publications.