Overview

  • Product name

    Anti-FUT8 antibody [EPR14149]
    See all FUT8 primary antibodies
  • Description

    Rabbit monoclonal [EPR14149] to FUT8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FUT8 aa 1-100. The exact sequence is proprietary.
    Database link: Q9BYC5

  • Positive control

    • HeLa and HepG2 whole cell lysate (ab7900)
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab191571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 66 kDa.
IP 1/140.

Target

  • Function

    Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.
  • Pathway

    Protein modification; protein glycosylation.
  • Sequence similarities

    Belongs to the glycosyltransferase 23 family.
    Contains 1 SH3 domain.
  • Cellular localization

    Golgi apparatus > Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi.
  • Information by UniProt
  • Database links

  • Form

    The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. There are 2 isoforms produced by alternative splicing. Immunogen is not present in isoform 2 (also known as Retinal).
  • Alternative names

    • 6-fucosyltransferase antibody
    • Alpha (1,6) fucosyltransferase antibody
    • Alpha-(1,6)-fucosyltransferase antibody
    • Alpha1 6FucT antibody
    • alpha1-6FucT antibody
    • Fucosyltransferase 8 (alpha (1,6) fucosyltransferase) antibody
    • Fucosyltransferase 8 antibody
    • FUT8 antibody
    • FUT8_HUMAN antibody
    • GDP fucose glycoprotein fucosyltransferase antibody
    • GDP L Fuc:N acetyl beta D glucosaminide alpha1,6 fucosyltransferase antibody
    • GDP-fucose--glycoprotein fucosyltransferase antibody
    • GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1 antibody
    • Glycoprotein 6 alpha L fucosyltransferase antibody
    • Glycoprotein 6-alpha-L-fucosyltransferase antibody
    • MGC26465 antibody
    see all

Images

  • Immunoprecipitation analysis of HeLa cell lysate labeling FUT8 using ab191571 at 1/140 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1500 was used as secondary antibody.

  • All lanes : Anti-FUT8 antibody [EPR14149] (ab191571) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 66 kDa



    5% NFDM/TBST blocking buffer

References

This product has been referenced in:

  • Fisher P  et al. Modeling Glycan Processing Reveals Golgi-Enzyme Homeostasis upon Trafficking Defects and Cellular Differentiation. Cell Rep 27:1231-1243.e6 (2019). Read more (PubMed: 31018136) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH3T3)
Gel Running Conditions
Reduced Denaturing
Loading amount
20000 cells
Specification
NIH3T3
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 18 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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