Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14149] to FUT8
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-FUT8 antibody [EPR14149]
See all FUT8 primary antibodies
DescriptionRabbit monoclonal [EPR14149] to FUT8
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human FUT8 aa 1-100. The exact sequence is proprietary.
Database link: Q9BYC5
- HeLa and HepG2 whole cell lysate (ab7900)
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab191571 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Predicted molecular weight: 66 kDa.|
FunctionCatalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the glycosyltransferase 23 family.
Contains 1 SH3 domain.
Cellular localizationGolgi apparatus > Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi.
- Information by UniProt
FormThe expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. There are 2 isoforms produced by alternative splicing. Immunogen is not present in isoform 2 (also known as Retinal).
- 6-fucosyltransferase antibody
- Alpha (1,6) fucosyltransferase antibody
- Alpha-(1,6)-fucosyltransferase antibody
Immunoprecipitation analysis of HeLa cell lysate labeling FUT8 using ab191571 at 1/140 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1500 was used as secondary antibody.
All lanes : Anti-FUT8 antibody [EPR14149] (ab191571) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 66 kDa
5% NFDM/TBST blocking buffer
ab191571 has been referenced in 1 publication.
- Fisher P et al. Modeling Glycan Processing Reveals Golgi-Enzyme Homeostasis upon Trafficking Defects and Cellular Differentiation. Cell Rep 27:1231-1243.e6 (2019). PubMed: 31018136