Recombinant
RabMAb

Recombinant Anti-FUT8 antibody [EPR14149] - BSA and Azide free (ab232637)

Overview

  • Product name

    Anti-FUT8 antibody [EPR14149] - BSA and Azide free
    See all FUT8 primary antibodies
  • Description

    Rabbit monoclonal [EPR14149] to FUT8 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FUT8 aa 1-100. The exact sequence is proprietary.
    Database link: Q9BYC5

  • Positive control

    • WB: HeLa and HepG2 whole cell lysates.
  • General notes

    Ab232637 is the carrier-free version of ab191571. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232637 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 66 kDa.
IP Use at an assay dependent concentration.

Target

  • Function

    Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.
  • Pathway

    Protein modification; protein glycosylation.
  • Sequence similarities

    Belongs to the glycosyltransferase 23 family.
    Contains 1 SH3 domain.
  • Cellular localization

    Golgi apparatus > Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi.
  • Information by UniProt
  • Database links

  • Form

    The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. There are 2 isoforms produced by alternative splicing. Immunogen is not present in isoform 2 (also known as Retinal).
  • Alternative names

    • 6-fucosyltransferase antibody
    • Alpha (1,6) fucosyltransferase antibody
    • Alpha-(1,6)-fucosyltransferase antibody
    • Alpha1 6FucT antibody
    • alpha1-6FucT antibody
    • Fucosyltransferase 8 (alpha (1,6) fucosyltransferase) antibody
    • Fucosyltransferase 8 antibody
    • FUT8 antibody
    • FUT8_HUMAN antibody
    • GDP fucose glycoprotein fucosyltransferase antibody
    • GDP L Fuc:N acetyl beta D glucosaminide alpha1,6 fucosyltransferase antibody
    • GDP-fucose--glycoprotein fucosyltransferase antibody
    • GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1 antibody
    • Glycoprotein 6 alpha L fucosyltransferase antibody
    • Glycoprotein 6-alpha-L-fucosyltransferase antibody
    • MGC26465 antibody
    see all

Images

  • Immunoprecipitation analysis of HeLa cell lysate labeling FUT8 using ab191571 at 1/140 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1500 was used as secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191571).

  • All lanes : Anti-FUT8 antibody [EPR14149] (ab191571) at 1/5000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 66 kDa



    5% NFDM/TBST blocking buffer.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191571).

References

ab232637 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab232637.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up