Overview

  • Product name

  • Description

    Rabbit polyclonal to FUZ
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: LLRNFYTLVT STHFPPEPGP PEKTEDEVYQ AQLPRACYLV LGTEEPGTGV RLVALQLGLR RLLLLLSPQS PTHGLRSLAT HTLHALTP, corresponding to amino acids 329-416 of Human FUZ.

  • Positive control

    • Human smooth muscle tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Target

  • Function

    Probable planar cell polarity effector involved in cilium biogenesis. May regulate protein and membrane transport to the cilium. May regulate the morphogenesis of hair follicles which depends on functional primary cilia.
  • Sequence similarities

    Belongs to the fuzzy family.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ22688 antibody
    • fuz antibody
    • FUZZY_HUMAN antibody
    • Fy antibody
    • Protein fuzzy homolog antibody
    see all

Images

  • Immunocytochemistry/immunofluorescence analysis of HEK293 cells labelling FUZ with ab122742 shows positive staining in the cytoplasm.
  • ab122742 at 1/50 dilution staining FUZ in Paraffin-embedded Human smooth muscle tissue by Immunohistochemistry.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

References

This product has been referenced in:

  • He M  et al. In vitro study of FUZ as a novel potential therapeutic target in non-small-cell lung cancer. Life Sci 197:91-100 (2018). Read more (PubMed: 29421438) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting Abcam.

Under our Abpromise we guarantee both ab122778 and ab122742 to work in IF on human samples. If you were to purchase either of them and they were not to perform as stated, then we would be happy to either replace or refund these antibodies.

As to which one to choose, based on the information that I have available, I think both would work just was well for you as the other.

Please let me know if there is anything else I can help you with.

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