Anti-FUZ antibody (ab122742)
Key features and details
- Rabbit polyclonal to FUZ
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FUZ antibody
See all FUZ primary antibodies -
Description
Rabbit polyclonal to FUZ -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: LLRNFYTLVT STHFPPEPGP PEKTEDEVYQ AQLPRACYLV LGTEEPGTGV RLVALQLGLR RLLLLLSPQS PTHGLRSLAT HTLHALTP, corresponding to amino acids 329-416 of Human FUZ.
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Positive control
- Human smooth muscle tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Function
Probable planar cell polarity effector involved in cilium biogenesis. May regulate protein and membrane transport to the cilium. May regulate the morphogenesis of hair follicles which depends on functional primary cilia. -
Sequence similarities
Belongs to the fuzzy family. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 80199 Human
- Omim: 610622 Human
- SwissProt: Q9BT04 Human
- Unigene: 288800 Human
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Alternative names
- FLJ22688 antibody
- fuz antibody
- FUZZY_HUMAN antibody
see all
Images
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Immunocytochemistry/immunofluorescence analysis of HEK293 cells labelling FUZ with ab122742 shows positive staining in the cytoplasm.
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ab122742 at 1/50 dilution staining FUZ in Paraffin-embedded Human smooth muscle tissue by Immunohistochemistry.
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Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab122742 has been referenced in 1 publication.
- He M et al. In vitro study of FUZ as a novel potential therapeutic target in non-small-cell lung cancer. Life Sci 197:91-100 (2018). PubMed: 29421438