Recombinant
RabMAb

Anti-FXL19 antibody [EPR11957] (ab172961)

Overview

  • Product name
    Anti-FXL19 antibody [EPR11957]
    See all FXL19 primary antibodies
  • Description
    Rabbit monoclonal [EPR11957] to FXL19
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FXL19 aa 500-600 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q6PCT2

  • Positive control
    • HT1080, A549 and mouse testis lysates; A549 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11957
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 76 kDa.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-FXL19 antibody [EPR11957] (ab172961) at 1/1000 dilution

      Lane 1 : HT1080 lysate
      Lane 2 : A549 lysate
      Lane 3 : Mouse testis lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 76 kDa

    • Immunofluorescence analysis of A549 cells labeling FXL19 with ab172961 at 1/50 dilution.

    References

    ab172961 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (hEK293)
    Total protein in input
    300 µg
    Immuno-precipitation step
    Other - protein G dynabeads
    Specification
    hEK293
    Username

    Dr. Edyta Marcon

    Verified customer

    Submitted Jun 27 2015

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10 % GEL, SDS)
    Sample
    Human Cell lysate - whole cell (HEK293, HELA, HCT116)
    Specification
    HEK293, HELA, HCT116
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 18 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up