Anti-FXR1 antibody (ab155124)
Key features and details
- Rabbit polyclonal to FXR1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human, Zebrafish
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-FXR1 antibody
See all FXR1 primary antibodies -
Description
Rabbit polyclonal to FXR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, Zebrafish
Predicted to work with: Chicken, Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 77-378 of Human FXR1 (UniProt: P51114).
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Positive control
- HepG2, HeLa and Mouse liver whole cell lysates; HeLa cells, Human CAL27 xenograft tissue; Zebrafish brain whole cell lysate and kidney tube
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 70 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 70 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs. -
Tissue specificity
Expressed in all tissues examined including heart, brain, kidney and testis. -
Sequence similarities
Belongs to the FMR1 family.
Contains 2 KH domains. -
Post-translational
modificationsArg-445 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 424968 Chicken
- Entrez Gene: 536793 Cow
- Entrez Gene: 8087 Human
- Entrez Gene: 14359 Mouse
- Entrez Gene: 327220 Zebrafish
- Omim: 600819 Human
- SwissProt: Q2TBT7 Cow
- SwissProt: P51114 Human
see all -
Alternative names
- Fragile X mental retardation autosomal homolog 1 antibody
- Fragile X mental retardation syndrome related protein 1 antibody
- Fragile X mental retardation syndrome-related protein 1 antibody
see all
Images
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Anti-FXR1 antibody (ab155124) at 1/1000 dilution + Zebrafish brain whole cell lysate at 30 µg
Predicted band size: 70 kDa7.5% SDS PAGE
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Anti-FXR1 antibody (ab155124) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 70 kDa
7.5% SDS PAGE -
Anti-FXR1 antibody (ab155124) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 70 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded sections of zebrafish kidney tube, using FXR1 antibody (ab155124) at 1:300 dilution.
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Immunofluorescent analysis of methanol-fixed HeLa cells, labeling FXR1 with ab155124 at 1/100 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded Human CAL27 xenograft tissue, labeling FXR1 with ab155124 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab155124 has been referenced in 5 publications.
- Jiang T et al. Linderae Radix Ethanol Extract Alleviates Diet-Induced Hyperlipidemia by Regulating Bile Acid Metabolism Through gut Microbiota. Front Pharmacol 12:627920 (2021). PubMed: 33679408
- Wang B et al. circNRIP1 facilitates keloid progression via FXR1-mediated upregulation of miR-503-3p and miR-503-5p. Int J Mol Med 47:N/A (2021). PubMed: 33649815
- Kaur H et al. Liver Bile Acid Changes in Mouse Models of Alzheimer's Disease. Int J Mol Sci 22:N/A (2021). PubMed: 34299071
- Feng X et al. Depletion of hepatic forkhead box O1 does not affect cholelithiasis in male and female mice. J Biol Chem 295:7003-7017 (2020). PubMed: 32273342
- Zhou H et al. Activation of FXR promotes intestinal metaplasia of gastric cells via SHP-dependent upregulation of the expression of CDX2. Oncol Lett 15:7617-7624 (2018). PubMed: 29849798