Overview

  • Product name
  • Description
    Rabbit polyclonal to FXR1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 77-378 of Human FXR1 (UniProt: P51114).

  • Positive control
    • HepG2, HeLa and Mouse liver whole cell lysates; HeLa cells, Human CAL27 xenograft tissue.
  • General notes
    Store as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 70 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs.
  • Tissue specificity
    Expressed in all tissues examined including heart, brain, kidney and testis.
  • Sequence similarities
    Belongs to the FMR1 family.
    Contains 2 KH domains.
  • Post-translational
    modifications
    Arg-445 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fragile X mental retardation autosomal homolog 1 antibody
    • Fragile X mental retardation syndrome related protein 1 antibody
    • Fragile X mental retardation syndrome-related protein 1 antibody
    • FXR1 antibody
    • FXR1_HUMAN antibody
    • FXR1P antibody
    • hFXR1p antibody
    see all

Images

  • Anti-FXR1 antibody (ab155124) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 70 kDa



    7.5% SDS PAGE
  • Anti-FXR1 antibody (ab155124) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg

    Predicted band size: 70 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells, labeling FXR1 with ab155124 at 1/100 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human CAL27 xenograft tissue, labeling FXR1 with ab155124 at 1/100 dilution.

References

This product has been referenced in:
  • Zhou H  et al. Activation of FXR promotes intestinal metaplasia of gastric cells via SHP-dependent upregulation of the expression of CDX2. Oncol Lett 15:7617-7624 (2018). Read more (PubMed: 29849798) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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