Anti-FXR1 antibody (ab245624)
Key features and details
- Rabbit polyclonal to FXR1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FXR1 antibody
See all FXR1 primary antibodies -
Description
Rabbit polyclonal to FXR1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human FXR1 aa 571-621. The exact sequence is proprietary. NP_005078.2
Database link: P51114 -
Positive control
- WB: HEK-293T, HeLa, 3T3 and Jurkat whole cell lysate. IP: HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab245624 was affinity purified using an epitope specific to FXR1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 70 kDa.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 70 kDa. |
Target
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Function
RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs. -
Tissue specificity
Expressed in all tissues examined including heart, brain, kidney and testis. -
Sequence similarities
Belongs to the FMR1 family.
Contains 2 KH domains. -
Post-translational
modificationsArg-445 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 8087 Human
- Entrez Gene: 14359 Mouse
- Omim: 600819 Human
- SwissProt: P51114 Human
- SwissProt: Q61584 Mouse
- Unigene: 478407 Human
- Unigene: 259021 Mouse
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Alternative names
- Fragile X mental retardation autosomal homolog 1 antibody
- Fragile X mental retardation syndrome related protein 1 antibody
- Fragile X mental retardation syndrome-related protein 1 antibody
see all
Images
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FXR1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) using ab245624 at 6 μg/reaction. Western blot was perfromed on the immunoprecipitate using ab245624 at 1 μg/ml.
Lane 1: ab245624 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds. -
All lanes : Anti-FXR1 antibody (ab245624) at 0.4 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 70 kDa
Exposure time: 3 minutes -
All lanes : Anti-FXR1 antibody (ab245624) at 0.1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 70 kDa
Exposure time: 3 minutes
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab245624 has not yet been referenced specifically in any publications.