Overview

  • Product name

  • Description

    Rabbit polyclonal to FXR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human FXR1 aa 571-621. The exact sequence is proprietary. NP_005078.2
    Database link: P51114

  • Positive control

    • WB: HEK-293T, HeLa, 3T3 and Jurkat whole cell lysate. IP: HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245624 was affinity purified using an epitope specific to FXR1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 70 kDa.

Target

  • Function

    RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs.
  • Tissue specificity

    Expressed in all tissues examined including heart, brain, kidney and testis.
  • Sequence similarities

    Belongs to the FMR1 family.
    Contains 2 KH domains.
  • Post-translational
    modifications

    Arg-445 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Fragile X mental retardation autosomal homolog 1 antibody
    • Fragile X mental retardation syndrome related protein 1 antibody
    • Fragile X mental retardation syndrome-related protein 1 antibody
    • FXR1 antibody
    • FXR1_HUMAN antibody
    • FXR1P antibody
    • hFXR1p antibody
    see all

Images

  • FXR1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) using ab245624 at 6 μg/reaction. Western blot was perfromed on the immunoprecipitate using ab245624 at 1 μg/ml.
     
    Lane 1: ab245624 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

  • All lanes : Anti-FXR1 antibody (ab245624) at 0.4 µg/ml

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 15 µg

    Developed using the ECL technique.

    Predicted band size: 70 kDa


    Exposure time: 3 minutes
  • All lanes : Anti-FXR1 antibody (ab245624) at 0.1 µg/ml

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 70 kDa


    Exposure time: 3 minutes

References

ab245624 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab245624.
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