Anti-FXR1 antibody (ab51970)
Key features and details
- Goat polyclonal to FXR1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FXR1 antibody
See all FXR1 primary antibodies -
Description
Goat polyclonal to FXR1 -
Host species
Goat -
Specificity
This antibody is expected to recognise all reported isoforms -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Dog
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Immunogen
Synthetic peptide:
RIEGDNENKLPRED
, corresponding to amino acids 317/330 of Human FXR1 -
Positive control
- WB: NIH/3T3 cell lysate. ICC: HeLa and A431 cells. IHC-P: Human skin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| WB | (2) |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).
1 hour primary incubation is recommended for this product. |
| Notes |
|---|
|
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs. -
Tissue specificity
Expressed in all tissues examined including heart, brain, kidney and testis. -
Sequence similarities
Belongs to the FMR1 family.
Contains 2 KH domains. -
Post-translational
modificationsArg-445 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 8087 Human
- Entrez Gene: 14359 Mouse
- Entrez Gene: 361927 Rat
- Omim: 600819 Human
- SwissProt: P51114 Human
- SwissProt: Q61584 Mouse
- SwissProt: Q5XI81 Rat
- Unigene: 478407 Human
see all -
Alternative names
- Fragile X mental retardation autosomal homolog 1 antibody
- Fragile X mental retardation syndrome related protein 1 antibody
- Fragile X mental retardation syndrome-related protein 1 antibody
see all
Images
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Western blot - Anti-FXR1 antibody (ab51970)Anti-FXR1 antibody (ab51970) at 0.2 µg/ml + NIH/3T3 cell lysate in RIPA buffer at 35 µg
Predicted band size: 69 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Immunocytochemistry - Anti-FXR1 antibody (ab51970)Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Immunocytochemistry - Anti-FXR1 antibody (ab51970)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FXR1 antibody (ab51970)IHC image of ab51970 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51970, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry/ Immunofluorescence - Anti-FXR1 antibody (ab51970)ICC/IF image of ab51970 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51970, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab51970 has been referenced in 5 publications.
- Zhao L et al. A Clostridia-rich microbiota enhances bile acid excretion in diarrhea-predominant irritable bowel syndrome. J Clin Invest 130:438-450 (2020). PubMed: 31815740
- Nordio L et al. Immunohistochemical Expression of FXR1 in Canine Normal Tissues and Melanomas. J Histochem Cytochem N/A:22155418766292 (2018). PubMed: 29608406
- Oldenburg AR et al. Deregulation of Fragile X-related protein 1 by the lipodystrophic lamin A p.R482W mutation elicits a myogenic gene expression program in preadipocytes. Hum Mol Genet N/A:N/A (2013). ICC/IF ; Human . PubMed: 24108105
- Xu XL et al. FXR1P But Not FMRP Regulates the Levels of Mammalian Brain-Specific microRNA-9 and microRNA-124. J Neurosci 31:13705-9 (2011). PubMed: 21957233
- Khera TK et al. Fragile X-related protein FXR1 controls post-transcriptional suppression of lipopolysaccharide-induced tumour necrosis factor-alpha production by transforming growth factor-beta1. FEBS J 277:2754-65 (2010). WB ; Mouse . PubMed: 20491901