Product nameAnti-FXR1 antibody [EPR7932]
See all FXR1 primary antibodies
DescriptionRabbit monoclonal [EPR7932] to FXR1
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues near the N-terminus of Human FXR1 (UniProt ID: P51114).
- Fetal muscle, MCF7, 293T, HeLa, and fetal heart lysates, Human brain tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.30 x 10 -12 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 70-78 kDa (predicted molecular weight: 70 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||Use at an assay dependent concentration.|
|ICC/IF||1/250 - 1/500.|
FunctionRNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs.
Tissue specificityExpressed in all tissues examined including heart, brain, kidney and testis.
Sequence similaritiesBelongs to the FMR1 family.
Contains 2 KH domains.
modificationsArg-445 is dimethylated, probably to asymmetric dimethylarginine.
- Information by UniProt
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All lanes : Anti-FXR1 antibody [EPR7932] (ab129089) at 1/1000 dilution
Lane 1 : Fetal muscle lysate
Lane 2 : MCF7 lysate
Lane 3 : 293T lysate
Lane 4 : HeLa lysate
Lane 5 : Fetal heart lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 70 kDa
Observed band size: 70-78 kDa why is the actual band size different from the predicted?
Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling FXR1 with purified ab129089 at 1/500 dilution. Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG (Alexa Fluor®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
ab129089 staining FXR1 in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/60. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
ab129089, at a dilution of 1/100, staining FXR1 in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab129089, at a dilution of 1/250, staining Hela cells by immunofluorescent staining
Equilibrium disassociation constant (KD)
Learn more about KD
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This product has been referenced in:
- Li P et al. Farnesoid X receptor interacts with cAMP response element binding protein to modulate glucagon-like peptide-1 (7-36) amide secretion by intestinal L cell. J Cell Physiol 234:12839-12846 (2019). Read more (PubMed: 30536761) »
- Li P et al. A novel epigenetic mechanism of FXR inhibiting GLP-1 secretion via miR-33 and its downstream targets. Biochem Biophys Res Commun 517:629-635 (2019). Read more (PubMed: 31387746) »