Overview

  • Product name

    Anti-FXR1 antibody [EPR7932]
    See all FXR1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7932] to FXR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of Human FXR1 (UniProt ID: P51114).

  • Positive control

    • Fetal muscle, MCF7, 293T, HeLa, and fetal heart lysates, Human brain tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 70-78 kDa (predicted molecular weight: 70 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs.
    • Tissue specificity

      Expressed in all tissues examined including heart, brain, kidney and testis.
    • Sequence similarities

      Belongs to the FMR1 family.
      Contains 2 KH domains.
    • Post-translational
      modifications

      Arg-445 is dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Fragile X mental retardation autosomal homolog 1 antibody
      • Fragile X mental retardation syndrome related protein 1 antibody
      • Fragile X mental retardation syndrome-related protein 1 antibody
      • FXR1 antibody
      • FXR1_HUMAN antibody
      • FXR1P antibody
      • hFXR1p antibody
      see all

    Images

    • All lanes : Anti-FXR1 antibody [EPR7932] (ab129089) at 1/1000 dilution

      Lane 1 : Fetal muscle lysate
      Lane 2 : MCF7 lysate
      Lane 3 : 293T lysate
      Lane 4 : HeLa lysate
      Lane 5 : Fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 70 kDa
      Observed band size: 70-78 kDa
      why is the actual band size different from the predicted?

    • Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling FXR1 with purified ab129089 at 1/500 dilution. Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG (Alexa Fluor®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • ab129089 staining FXR1 in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/60. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

      Isoytype control: Rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    • ab129089, at a dilution of 1/100, staining FXR1 in paraffin-embedded Human brain tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab129089, at a dilution of 1/250, staining Hela cells by immunofluorescent staining
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Li P  et al. Farnesoid X receptor interacts with cAMP response element binding protein to modulate glucagon-like peptide-1 (7-36) amide secretion by intestinal L cell. J Cell Physiol 234:12839-12846 (2019). Read more (PubMed: 30536761) »
    • Li P  et al. A novel epigenetic mechanism of FXR inhibiting GLP-1 secretion via miR-33 and its downstream targets. Biochem Biophys Res Commun 517:629-635 (2019). Read more (PubMed: 31387746) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Neuro2a cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Neuro2a cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C

    Dr. Alexandre Magno

    Verified customer

    Submitted Jun 26 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up