Recombinant Anti-FXYD1/PLM antibody [EPR16081] (ab191397)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16081] to FXYD1/PLM
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-FXYD1/PLM antibody [EPR16081]
See all FXYD1/PLM primary antibodies -
Description
Rabbit monoclonal [EPR16081] to FXYD1/PLM -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human skeletal muscle lysate; Human fetal heart and fetal liver lysates; Human cardiac muscle tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16081 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/350 - 1/700. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/90.
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WB |
1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).
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Notes |
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IHC-P
1/350 - 1/700. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/90. |
WB
1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa). |
Target
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Function
May have a functional role in muscle contraction. Induces a hyperpolarization-activated chloride current when exogenously expressed. -
Tissue specificity
Highest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining. Very low levels in kidney, colon and small intestine without mucosa, prostate without endothelial lining, spleen, and testis. -
Sequence similarities
Belongs to the FXYD family. -
Post-translational
modificationsMajor plasma membrane substrate for cAMP-dependent protein kinase (PK-A) and protein kinase C (PK-C) in several different tissues (By similarity). Phosphorylated in response to insulin and adrenergic stimulation. May be phosphorylated by DMPK.
Palmitoylation increases half-life and stability, it is enhanced upon phosphorylation at Ser-88 by PKA. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5348 Human
- Omim: 602359 Human
- SwissProt: O00168 Human
- Unigene: 442498 Human
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Alternative names
- FXYD domain containing ion transport regulator 1 antibody
- FXYD domain-containing ion transport regulator 1 antibody
- FXYD1 antibody
see all
Images
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All lanes : Anti-FXYD1/PLM antibody [EPR16081] (ab191397) at 1/10000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human fetal liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 10 kDa -
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling FXYD1/PLM with ab191397 at 1/700 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Inset: Negative control: using PBS instead of primary antibody.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Western blot analysis of immunoprecipitation pellet from Human fetal livert lysate immunoprecipitated using ab191397 at 1/90 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab191397 has not yet been referenced specifically in any publications.