Product nameAnti-FXYD1/PLM antibody
See all FXYD1/PLM primary antibodies
DescriptionRabbit polyclonal to FXYD1/PLM
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Macaque monkey
Synthetic peptide corresponding to Human FXYD1/PLM aa 50 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following tissue lysates: Human Brain; Human Brain Membrane; Human Heart; Human Skeletal Muscle
This product was previously labelled as FXYD1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab76597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionMay have a functional role in muscle contraction. Induces a hyperpolarization-activated chloride current when exogenously expressed.
Tissue specificityHighest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining. Very low levels in kidney, colon and small intestine without mucosa, prostate without endothelial lining, spleen, and testis.
Sequence similaritiesBelongs to the FXYD family.
modificationsMajor plasma membrane substrate for cAMP-dependent protein kinase (PK-A) and protein kinase C (PK-C) in several different tissues (By similarity). Phosphorylated in response to insulin and adrenergic stimulation. May be phosphorylated by DMPK.
Palmitoylation increases half-life and stability, it is enhanced upon phosphorylation at Ser-88 by PKA.
- Information by UniProt
- FXYD domain containing ion transport regulator 1 antibody
- FXYD domain-containing ion transport regulator 1 antibody
- FXYD1 antibody
All lanes : Anti-FXYD1/PLM antibody (ab76597) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human brain normal tissue lysate - membrane extract (ab29456)
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 10 kDa
Observed band size: 10 kDa
Exposure time: 5 minutes
ab76597 at a 1/100 dilution, staining mouse FXYD1/PLM in heart and muscle tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.
ab76597 has been referenced in 6 publications.
- Ke HY et al. Changes in cellular Ca2+ and Na+ regulation during the progression towards heart failure in the guinea pig. J Physiol N/A:N/A (2019). PubMed: 30811606
- Simon MJ et al. Transcriptional network analysis of human astrocytic endfoot genes reveals region-specific associations with dementia status and tau pathology. Sci Rep 8:12389 (2018). PubMed: 30120299
- Wanichawan P et al. Development of a high-affinity peptide that prevents phospholemman (PLM) inhibition of the sodium/calcium exchanger 1 (NCX1). Biochem J 473:2413-23 (2016). WB ; Rat . PubMed: 27247424
- Hafver TL et al. Protein Phosphatase 1c Associated with the Cardiac Sodium Calcium Exchanger 1 Regulates Its Activity by Dephosphorylating Serine 68-phosphorylated Phospholemman. J Biol Chem 291:4561-79 (2016). PubMed: 26668322
- Howie J et al. Substrate recognition by the cell surface palmitoyl transferase DHHC5. Proc Natl Acad Sci U S A 111:17534-9 (2014). PubMed: 25422474
- Matagne V et al. Correcting deregulated Fxyd1 expression ameliorates a behavioral impairment in a mouse model of Rett syndrome. Brain Res 1496:104-14 (2013). WB ; Mouse . PubMed: 23246925