Product nameAnti-FXYD1/PLM antibody [EPR16081]
See all FXYD1/PLM primary antibodies
DescriptionRabbit monoclonal [EPR16081] to FXYD1/PLM
Tested applicationsSuitable for: IHC-P, IP, WBmore details
Species reactivityReacts with: Human
within Human FXYD1/PLM aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: O00168
- Human skeletal muscle lysate; Human fetal heart and fetal liver lysates; Human cardiac muscle tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab191397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/350 - 1/700. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).|
FunctionMay have a functional role in muscle contraction. Induces a hyperpolarization-activated chloride current when exogenously expressed.
Tissue specificityHighest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining. Very low levels in kidney, colon and small intestine without mucosa, prostate without endothelial lining, spleen, and testis.
Sequence similaritiesBelongs to the FXYD family.
modificationsMajor plasma membrane substrate for cAMP-dependent protein kinase (PK-A) and protein kinase C (PK-C) in several different tissues (By similarity). Phosphorylated in response to insulin and adrenergic stimulation. May be phosphorylated by DMPK.
Palmitoylation increases half-life and stability, it is enhanced upon phosphorylation at Ser-88 by PKA.
- Information by UniProt
- FXYD domain containing ion transport regulator 1 antibody
- FXYD domain-containing ion transport regulator 1 antibody
- FXYD1 antibody
All lanes : Anti-FXYD1/PLM antibody [EPR16081] (ab191397) at 1/10000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal liver lysate
Lysates/proteins at 20 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 10 kDa
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling FXYD1/PLM with ab191397 at 1/700 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Inset: Negative control: using PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot analysis of immunoprecipitation pellet from Human fetal livert lysate immunoprecipitated using ab191397 at 1/90 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
ab191397 has not yet been referenced specifically in any publications.