Overview

  • Product name

    Anti-FXYD1/PLM antibody [EPR16081]
    See all FXYD1/PLM primary antibodies
  • Description

    Rabbit monoclonal [EPR16081] to FXYD1/PLM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    within Human FXYD1/PLM aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: O00168

  • Positive control

    • Human skeletal muscle lysate; Human fetal heart and fetal liver lysates; Human cardiac muscle tissue.
  • General notes

     

     

     This product was previously labelled as FXYD1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab191397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/350 - 1/700. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/90.
WB 1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).

Target

  • Function

    May have a functional role in muscle contraction. Induces a hyperpolarization-activated chloride current when exogenously expressed.
  • Tissue specificity

    Highest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining. Very low levels in kidney, colon and small intestine without mucosa, prostate without endothelial lining, spleen, and testis.
  • Sequence similarities

    Belongs to the FXYD family.
  • Post-translational
    modifications

    Major plasma membrane substrate for cAMP-dependent protein kinase (PK-A) and protein kinase C (PK-C) in several different tissues (By similarity). Phosphorylated in response to insulin and adrenergic stimulation. May be phosphorylated by DMPK.
    Palmitoylation increases half-life and stability, it is enhanced upon phosphorylation at Ser-88 by PKA.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FXYD domain containing ion transport regulator 1 antibody
    • FXYD domain-containing ion transport regulator 1 antibody
    • FXYD1 antibody
    • Phospholemman antibody
    • PLM antibody
    • PLM_HUMAN antibody
    see all

Images

  • All lanes : Anti-FXYD1/PLM antibody [EPR16081] (ab191397) at 1/10000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 10 kDa

  • Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling FXYD1/PLM with ab191397 at 1/700 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    Inset: Negative control: using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot analysis of immunoprecipitation pellet from Human fetal livert lysate immunoprecipitated using ab191397 at 1/90 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

References

ab191397 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191397.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up