Overview

  • Product name

    Anti-FXYD1/PLM antibody [EPR16082]
    See all FXYD1/PLM primary antibodies
  • Description

    Rabbit monoclonal [EPR16082] to FXYD1/PLM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human FXYD1/PLM aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: O00168

  • Positive control

    • WB: Human skeletal muscle, fetal heart and fetal liver lysates; Mouse and rat heart lysates. IP: Human fetal heart whole cell lysate.
  • General notes

     

     

     This product was previously labelled as FXYD1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab200204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).
IP 1/120.

Target

  • Function

    May have a functional role in muscle contraction. Induces a hyperpolarization-activated chloride current when exogenously expressed.
  • Tissue specificity

    Highest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining. Very low levels in kidney, colon and small intestine without mucosa, prostate without endothelial lining, spleen, and testis.
  • Sequence similarities

    Belongs to the FXYD family.
  • Post-translational
    modifications

    Major plasma membrane substrate for cAMP-dependent protein kinase (PK-A) and protein kinase C (PK-C) in several different tissues (By similarity). Phosphorylated in response to insulin and adrenergic stimulation. May be phosphorylated by DMPK.
    Palmitoylation increases half-life and stability, it is enhanced upon phosphorylation at Ser-88 by PKA.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FXYD domain containing ion transport regulator 1 antibody
    • FXYD domain-containing ion transport regulator 1 antibody
    • FXYD1 antibody
    • Phospholemman antibody
    • PLM antibody
    • PLM_HUMAN antibody
    see all

Images

  • Anti-FXYD1/PLM antibody [EPR16082] (ab200204) at 1/1000 dilution + Human skeletal muscle lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 12 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-FXYD1/PLM antibody [EPR16082] (ab200204) at 1/10000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 12 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-FXYD1/PLM antibody [EPR16082] (ab200204) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 12 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • FXYD1/PLM was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab200204 at 1/120 dilution. Western blot was performed using ab200204 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human fetal heart whole cell lysate 10ug (Input).

    Lane 2: ab200204 IP in Human fetal heart whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200204 in Human fetal heart whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

This product has been referenced in:

  • Eleftheriadou O  et al. Expression and regulation of type 2A protein phosphatases and alpha4 signalling in cardiac health and hypertrophy. Basic Res Cardiol 112:37 (2017). Rat . Read more (PubMed: 28526910) »
See 1 Publication for this product

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