Recombinant
RabMAb

Recombinant Anti-FXYD6/Plp antibody [EPR9004(2)] (ab181254)

Overview

  • Product name

    Anti-FXYD6/Plp antibody [EPR9004(2)]
    See all FXYD6/Plp primary antibodies
  • Description

    Rabbit monoclonal [EPR9004(2)] to FXYD6/Plp
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human FXYD6/Plp aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9H0Q3

  • Positive control

    • Human testis and Human fetal brain lysates.
  • General notes

     

     

     This product was previously labelled as FXYD6

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9004(2)
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab181254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50 - 1/80.
WB 1/1000 - 1/50000. Detects a band of approximately 18 kDa (predicted molecular weight: 11 kDa).

Target

Images

  • Anti-FXYD6/Plp antibody [EPR9004(2)] (ab181254) at 1/50000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?

  • Anti-FXYD6/Plp antibody [EPR9004(2)] (ab181254) at 1/10000 dilution + Human testis lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa
    Observed band size: 18 kDa why is the actual band size different from the predicted?

  • Western blot analysis on immunoprecipitation from Human testis lysate labeling FXYD6/Plp using ab181254 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1000 dilution.

References

ab181254 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181254.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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