Recombinant Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free (ab250467)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9004(2)] to FXYD6/Plp - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free
See all FXYD6/Plp primary antibodies -
Description
Rabbit monoclonal [EPR9004(2)] to FXYD6/Plp - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250467 is the carrier-free version of ab181254.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9004(2) -
Isotype
IgG
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 11 kDa).
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Notes |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 11 kDa). |
Target
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Relevance
FXYD6 is a putative ion transporter regulator that is associated with susceptibility to schizophrenia type 2 (SCZD2). -
Cellular localization
Plasma membrane -
Database links
- Entrez Gene: 53826 Human
- Entrez Gene: 59095 Mouse
- Entrez Gene: 63847 Rat
- Omim: 606683 Human
- SwissProt: Q9H0Q3 Human
- SwissProt: Q9D164 Mouse
- SwissProt: Q91XV6 Rat
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Alternative names
- FXYD domain containing ion transport regulator 6 antibody
- FXYD domain containing ion transport regulator 6 precursor antibody
- Phosphohippolin antibody
- UNQ521/PRO1056 antibody
Images
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This data was developed using ab250467, the same antibody clone in a different buffer formulation.
Purified ab250467 at 1:20 dilution (0.8µg) immunoprecipitating FXYD6/Plp in Human testis lysate.
Lane 1 (input): Human testis lysate 10 ?g
Lane 2 (+): ab250467 + Human testis lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181254 in human testis lysate.
VeriBlot for IP Detection Reagent (HRP)(ab131366) (1:1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250467 has not yet been referenced specifically in any publications.