Product nameAnti-FYB antibody [EP2546Y]
See all FYB primary antibodies
DescriptionRabbit monoclonal [EP2546Y] to FYB
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human FYB aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: O15117
- TF-1 lysate. Human tonsil tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/5000 - 1/10000. Predicted molecular weight: 85 kDa.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionActs as an adapter protein of the FYN and LCP2 signaling cascades in T cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2.
Tissue specificityExpressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues.
Sequence similaritiesContains 1 SH3 domain.
modificationsT-cell receptor ligation leads to increased tyrosine phosphorylation.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- ADAP antibody
- Adhesion and degranulation promoting adaptor protein antibody
- FYB 120/130 antibody
Anti-FYB antibody [EP2546Y] (ab76103) at 1/10000 dilution + TF-1 lysate at 10 µg
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 85 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
ab76103, at 1/250 dilution, staining FYB in paraffin-embedded human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/If image of CHO cell expressing human ADAP using ab 76103. The cells were fixed in formaldehyde and permeabilised using 0.2% Triton X100 for 5 mins. The cells were them blocked using 10% serum for 1 hour.
ab76103 has not yet been referenced specifically in any publications.