Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2547Y] to FYB
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Product nameAnti-FYB antibody [EPR2547Y]
See all FYB primary antibodies
DescriptionRabbit monoclonal [EPR2547Y] to FYB
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human FYB aa 700-800 (C terminal). The exact sequence is proprietary.
- TF-1 lysate. Human tonsil tissue.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
WB: 1/500. Predicted molecular weight: 85 kDa.
Is unsuitable for Flow Cyt or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionActs as an adapter protein of the FYN and LCP2 signaling cascades in T cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2.
Tissue specificityExpressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues.
Sequence similaritiesContains 1 SH3 domain.
modificationsT-cell receptor ligation leads to increased tyrosine phosphorylation.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- ADAP antibody
- Adhesion and degranulation promoting adaptor protein antibody
- FYB 120/130 antibody
Anti-FYB antibody [EPR2547Y] (ab76006) at 1/500 dilution + TF-1 lysate at 10 µg
Goat anti-rabbit HRP at 1/1000 dilution
Predicted band size: 85 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
ab76006, at 1/100 dilution, staining FYB in human tonsil by immunohistochemistry using paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab76006 has been referenced in 1 publication.
- Wu Z et al. Fyn-binding protein ADAP supports actin organization in podocytes. Physiol Rep 5:N/A (2017). WB ; Mouse . PubMed: 29192064