Platelet lysate (not shown) Human ADAP Transfection
Human ADAP lacking COOH terminus (ADAP d COOH)
We transfected CHO cells with a construct expressing wild-type ADAP or ADAP lacking the COOH terminus. The Abcam antibody gave a clean band of the correct size for the wild type (shown in green), but no band for the mutant as expected, showing the antibody specificity. The blot wass reprobed for b-actin, as a loading control.
Abcam user community
Submitted Jun 20 2012
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