Western blot abreview for Anti-FYB antibody [EPR2547Y]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (CHO cell transfected)
Loading amount
20 µg
Specification
CHO cell transfected
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

Other product details

Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBS_T

Secondary antibody

Name
Non-Abcam antibody was used: Odyssey 800
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Licor
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 130 kDa
Positive control
Platelet lysate (not shown)
Human ADAP Transfection
Negative control
Human ADAP lacking COOH terminus (ADAP d COOH)

Additional data

Additional Notes
We transfected CHO cells with a construct expressing wild-type ADAP or ADAP lacking the COOH terminus. The Abcam antibody gave a clean band of the correct size for the wild type (shown in green), but no band for the mutant as expected, showing the antibody specificity. The blot wass reprobed for b-actin, as a loading control.

Abcam user community

Verified customer

Submitted Jun 20 2012

Sign up