Recombinant Anti-FYB antibody [EPR2547Y] - BSA and Azide free (ab247426)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-FYB antibody [EPR2547Y] - BSA and Azide free
See all FYB primary antibodies -
Description
Rabbit monoclonal [EPR2547Y] to FYB - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human FYB aa 700-800 (C terminal). The exact sequence is proprietary.
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General notes
ab247426 is the carrier-free version of ab76006 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247426 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR2547Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab247426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 85 kDa. |
Target
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Function
Acts as an adapter protein of the FYN and LCP2 signaling cascades in T cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2. -
Tissue specificity
Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues. -
Sequence similarities
Contains 1 SH3 domain. -
Post-translational
modificationsT-cell receptor ligation leads to increased tyrosine phosphorylation. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2533 Human
- Entrez Gene: 23880 Mouse
- Omim: 602731 Human
- SwissProt: O15117 Human
- SwissProt: O35601 Mouse
- Unigene: 370503 Human
- Unigene: 170905 Mouse
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Alternative names
- ADAP antibody
- Adhesion and degranulation promoting adaptor protein antibody
- FYB 120/130 antibody
see all
Datasheets and documents
References
ab247426 has not yet been referenced specifically in any publications.
