Product nameAnti-FYCO1 antibody
See all FYCO1 primary antibodies
DescriptionRabbit polyclonal to FYCO1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Dog, Chimpanzee, Orangutan
Synthetic peptide within Human FYCO1 aa 325-375. The exact sequence is proprietary. (NP_078789.2).
Database link: Q9BQS8
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab224818 was affinity purified using an epitope specific to FYCO1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab224818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 167 kDa.|
|IP||Use at 5-15 µg/mg of lysate.|
Tissue specificityExpressed in heart and skeletal muscle.
Sequence similaritiesContains 1 FYVE-type zinc finger.
Contains 1 GOLD domain.
Contains 1 RUN domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- CATC2 antibody
- CTRCT18 antibody
- DKFZp779K1152 antibody
All lanes : Anti-FYCO1 antibody (ab224818) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 167 kDa
Exposure time: 3 minutes
FYCO1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224818 at 10 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224818 at 0.4 µg/ml.
Lane 1: ab224818 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab224818 has not yet been referenced specifically in any publications.