Overview

  • Product name

  • Description

    Rabbit polyclonal to FYCO1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Dog, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide within Human FYCO1 aa 325-375. The exact sequence is proprietary. (NP_078789.2).
    Database link: Q9BQS8

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab224818 was affinity purified using an epitope specific to FYCO1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 167 kDa.
IP Use at 5-15 µg/mg of lysate.

Target

  • Tissue specificity

    Expressed in heart and skeletal muscle.
  • Sequence similarities

    Contains 1 FYVE-type zinc finger.
    Contains 1 GOLD domain.
    Contains 1 RUN domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links

  • Alternative names

    • CATC2 antibody
    • CTRCT18 antibody
    • DKFZp779K1152 antibody
    • FLJ13335 antibody
    • FYCO1 antibody
    • FYCO1_HUMAN antibody
    • FYVE and coiled coil domain containing 1 antibody
    • FYVE and coiled coil domain containing protein 1 antibody
    • FYVE and coiled-coil domain-containing protein 1 antibody
    • MGC126517 antibody
    • MGC126519 antibody
    • RUFY3 antibody
    • RUN and FYVE domain containing 3 antibody
    • ZFYVE7 antibody
    • Zinc finger FYVE domain containing protein 7 antibody
    • Zinc finger FYVE domain-containing protein 7 antibody
    see all

Images

  • All lanes : Anti-FYCO1 antibody (ab224818) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 167 kDa


    Exposure time: 3 minutes
  • FYCO1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224818 at 10 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224818 at 0.4 µg/ml.

    Lane 1: ab224818 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 30 seconds.

References

ab224818 has not yet been referenced specifically in any publications.

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