Overview

  • Product name

    Anti-Fyn antibody [2A10]
    See all Fyn primary antibodies
  • Description

    Mouse monoclonal [2A10] to Fyn
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Purified recombinant fragment of Human Fyn, expressed in E. coli.

  • Positive control

    • IHC-P: Human breast cancer and brain tissues ICC/IF: U251 cells Flow Cyt: Hela cells

Properties

Applications

Our Abpromise guarantee covers the use of ab119855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 61 kDa.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/200 - 1/1000.

Target

  • Function

    Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3.
  • Tissue specificity

    Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Cellular localization

    Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.
  • Information by UniProt
  • Database links

  • Form

    This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • Alternative names

    • C syn protooncogene antibody
    • Fyn antibody
    • FYN oncogene related to SRC FGR YES antibody
    • FYN_HUMAN antibody
    • OKT3 induced calcium influx regulator antibody
    • P59 FYN antibody
    • p59-Fyn antibody
    • Protein tyrosine kinase fyn antibody
    • Proto oncogene tyrosine protein kinase fyn antibody
    • Proto-oncogene c-Fyn antibody
    • Proto-oncogene Syn antibody
    • Protooncogene Syn antibody
    • SLK antibody
    • Src like kinase antibody
    • Src yes related novel gene antibody
    • Src-like kinase antibody
    • Src/yes related novel antibody
    • SYN antibody
    • Tyrosine kinase p59fyn T antibody
    • Tyrosine kinase p59fyn(T) antibody
    • Tyrosine-protein kinase Fyn antibody
    see all

Images

  • Anti-Fyn antibody [2A10] (ab119855) at 1/500 dilution + Fyn recombinant protein (7-176 amino acids)

    Predicted band size: 61 kDa



    Predicted MW of Recombinant Fragment : 44.3 kDa
  • ab119855, at a 1/200 dilution, staining Fyn in paraffin embedded Human breast cancer tissue by Immunohistochemistry, with DAB staining.
  • ab119855, at a 1/200 dilution, staining Fyn in paraffin embedded Human brain tissue by Immunohistochemistry, with DAB staining.
  • Immunofluorescence analysis of U251 cells, using ab119855 at a 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Flow Cytometric analysis of Hela cells, using ab119855 at a 1/200 dilution (green) and negative control (purple).
  • Elisa results for ab119855

References

This product has been referenced in:

  • Yamada Y  et al. Identification of hypo- and hypermethylated genes related to atherosclerosis by a genome-wide analysis of DNA methylation. Int J Mol Med 33:1355-63 (2014). Read more (PubMed: 24626634) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.
CCK4
ab104839 was generated against amino acids x-y.

RON
ab93129 was generated against a partial recombinant protein containing the protein sequence spanning 163-514. A his-tag was used in the protein.

ROR2
ab92379 The peptide maps between amino acids 40 and 65 of human ROR2 (UniProt: Q01974), i.e. an extracellular epitope is detected.
ab80174 The antigen is recorded in our system as a his-tag fusion protein, without specifying whether it is a partial or full-length recombinant protein. We apologise that more information is currently not available.

Fgr
ab81599 was generated against amino acids 2-100.
ab92302 The peptide maps between amino acids 25 and 50 of human FGR (UniProt: P09769)
ab65007 The amino acid range for this immunogen is 61-110 from human FGR (P09769).

Fer
ab109740 The recombinant protein used as the immunogen for this antibody encodes amino acids x-y.
Brk
ab52273 was generated against amino acids 2-175.

Fes
ab108418 The peptide maps between amino acids 760 and 790 of human FES (UniProt: P07332).

Frk
ab91623 was generated against amino acids x-y.

Fyn
ab119855 was generated against amino acids 7-176

Csk
ab125005 The peptide maps between amino acids 75 and 105 of human CSK (UniProt: P41240)

Lyn
ab52563 see attachment

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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