Key features and details
- Mouse monoclonal [2A10] to Fyn
- Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Fyn antibody [2A10]
See all Fyn primary antibodies
DescriptionMouse monoclonal [2A10] to Fyn
Tested applicationsSuitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Purified recombinant fragment of Human Fyn, expressed in E. coli.
- IHC-P: Human breast cancer and brain tissues ICC/IF: U251 cells Flow Cyt: Hela cells
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab119855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 61 kDa.|
|IHC-P||1/200 - 1/1000.|
|Flow Cyt||1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/200 - 1/1000.|
FunctionTyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3.
Tissue specificityIsoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
Cellular localizationCell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.
- Information by UniProt
FormThis protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
- C syn protooncogene antibody
- Fyn antibody
- FYN oncogene related to SRC FGR YES antibody
Anti-Fyn antibody [2A10] (ab119855) at 1/500 dilution + Fyn recombinant protein (7-176 amino acids)
Predicted band size: 61 kDa
Predicted MW of Recombinant Fragment : 44.3 kDa
ab119855, at a 1/200 dilution, staining Fyn in paraffin embedded Human breast cancer tissue by Immunohistochemistry, with DAB staining.
ab119855, at a 1/200 dilution, staining Fyn in paraffin embedded Human brain tissue by Immunohistochemistry, with DAB staining.
Immunofluorescence analysis of U251 cells, using ab119855 at a 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometric analysis of Hela cells, using ab119855 at a 1/200 dilution (green) and negative control (purple).
Elisa results for ab119855
ab119855 has been referenced in 1 publication.
- Yamada Y et al. Identification of hypo- and hypermethylated genes related to atherosclerosis by a genome-wide analysis of DNA methylation. Int J Mol Med 33:1355-63 (2014). PubMed: 24626634