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Anti-Fyn antibody (ab227304)

  • Datasheet
  • SDS
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Western blot - Anti-Fyn antibody (ab227304)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody (ab227304)
  • Western blot - Anti-Fyn antibody (ab227304)

Key features and details

  • Rabbit polyclonal to Fyn
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant human Fyn protein (ab84696)

View more associated products

Overview

  • Product name

    Anti-Fyn antibody
    See all Fyn primary antibodies
  • Description

    Rabbit polyclonal to Fyn
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Pig, Xenopus laevis
  • Immunogen

    Recombinant fragment within Human Fyn (internal sequence). The exact sequence is proprietary.
    Database link: P06241

  • Positive control

    • WB: Mouse and rat brain extracts; Jurkat whole cell lysate. IHC-P: Human colon carcinoma tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Src Family
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Jurkat whole cell lysate (ab30128)
    • Mouse brain tissue lysate - total protein (ab30151)
    • Mouse brain tissue lysate - total protein (ab4022)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant human Fyn protein (ab84696)
  • Related Products

    • Recombinant human Fyn protein (ab135007)

Applications

Our Abpromise guarantee covers the use of ab227304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 60 kDa.

Target

  • Function

    Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3.
  • Tissue specificity

    Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Cellular localization

    Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.
  • Target information above from: UniProt accession P06241 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 396294 Chicken
    • Entrez Gene: 527263 Cow
    • Entrez Gene: 2534 Human
    • Entrez Gene: 14360 Mouse
    • Entrez Gene: 791125 Pig
    • Entrez Gene: 25150 Rat
    • Entrez Gene: 373609 Xenopus laevis
    • Entrez Gene: 379812 Xenopus laevis
    • Omim: 137025 Human
    • SwissProt: Q05876 Chicken
    • SwissProt: A0JNB0 Cow
    • SwissProt: P06241 Human
    • SwissProt: P39688 Mouse
    • SwissProt: A1Y2K1 Pig
    • SwissProt: Q62844 Rat
    • SwissProt: P13406 Xenopus laevis
    • Unigene: 390567 Human
    • Unigene: 4848 Mouse
    • Unigene: 19361 Rat
    • Unigene: 21919 Xenopus laevis
    • Unigene: 501 Xenopus laevis
    see all
  • Form

    This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • Alternative names

    • C syn protooncogene antibody
    • Fyn antibody
    • FYN oncogene related to SRC FGR YES antibody
    • FYN_HUMAN antibody
    • OKT3 induced calcium influx regulator antibody
    • P59 FYN antibody
    • p59-Fyn antibody
    • Protein tyrosine kinase fyn antibody
    • Proto oncogene tyrosine protein kinase fyn antibody
    • Proto-oncogene c-Fyn antibody
    • Proto-oncogene Syn antibody
    • Protooncogene Syn antibody
    • SLK antibody
    • Src like kinase antibody
    • Src yes related novel gene antibody
    • Src-like kinase antibody
    • Src/yes related novel antibody
    • SYN antibody
    • Tyrosine kinase p59fyn T antibody
    • Tyrosine kinase p59fyn(T) antibody
    • Tyrosine-protein kinase Fyn antibody
    see all

Images

  • Western blot - Anti-Fyn antibody (ab227304)
    Western blot - Anti-Fyn antibody (ab227304)
    All lanes : Anti-Fyn antibody (ab227304) at 1/1000 dilution

    Lane 1 : Mouse brain extract
    Lane 2 : Rat brain extract

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 60 kDa



    10% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody (ab227304)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody (ab227304)

    Paraffin-embedded human colon carcinoma tissue stained for Fyn using ab227304 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-Fyn antibody (ab227304)
    Western blot - Anti-Fyn antibody (ab227304)
    Anti-Fyn antibody (ab227304) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

    Predicted band size: 60 kDa



    10% SDS-PAGE gel.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab227304? Please let us know so that we can cite the reference in this datasheet.

    ab227304 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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