- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Fyn antibody
See all Fyn primary antibodies -
DescriptionMouse monoclonal to Fyn
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Host speciesMouse
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Tested applicationsSuitable for: WB, ELISAmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant full length protein, corresponding to amino acids 1-483 of Human Fyn (AAH32496), with a 26kDa enzyme tag.
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Positive control
- FYN transfected 293T cell lysate
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General notes
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab125016 as a replacement.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: PBS, pH 7.2 -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab76934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). | |
| ELISA | Use at an assay dependent dilution. |
Target
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FunctionTyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3.
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Tissue specificityIsoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.
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Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Cellular localizationCell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.
- Information by UniProt
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Database links
- Entrez Gene: 2534 Human
- Omim: 137025 Human
- SwissProt: P06241 Human
- Unigene: 390567 Human
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FormThis protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
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Alternative names
- C syn protooncogene antibody
- Fyn antibody
- FYN oncogene related to SRC FGR YES antibody
see all
Images
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Western blot - Anti-Fyn antibody (ab76934)Anti-Fyn antibody (ab76934) at 5 µg/ml + Immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 60 kDa
Observed band size: 86 kDa (why is the actual band size different from the predicted?) -
Western blot - Anti-Fyn antibody (ab76934)All lanes : Anti-Fyn antibody (ab76934) at 5 µg/ml
Lane 1 : Transfected 293T cell lysate
Lane 2 : Untransfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Protocols
References
ab76934 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"