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Anti-Fyn antibody (ab76934)

  • Datasheet
  • SDS
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Western blot - Anti-Fyn antibody (ab76934)
  • Western blot - Anti-Fyn antibody (ab76934)

Key features and details

  • Mouse monoclonal to Fyn
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG1

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Protein
Product image
Recombinant human Fyn protein (ab84696)

View more associated products

Overview

  • Product name

    Anti-Fyn antibody
    See all Fyn primary antibodies
  • Description

    Mouse monoclonal to Fyn
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-483 of Human Fyn (AAH32496), with a 26kDa enzyme tag.

  • Positive control

    • FYN transfected 293T cell lysate
  • General notes

    This product was changed from ascites to tissue culture supernatant on 30th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.4
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Src Family
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant human Fyn protein (ab84696)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

Target

  • Function

    Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3.
  • Tissue specificity

    Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Cellular localization

    Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.
  • Target information above from: UniProt accession P06241 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2534 Human
    • Omim: 137025 Human
    • SwissProt: P06241 Human
    • Unigene: 390567 Human
    • Form

      This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
    • Alternative names

      • C syn protooncogene antibody
      • Fyn antibody
      • FYN oncogene related to SRC FGR YES antibody
      • FYN_HUMAN antibody
      • OKT3 induced calcium influx regulator antibody
      • P59 FYN antibody
      • p59-Fyn antibody
      • Protein tyrosine kinase fyn antibody
      • Proto oncogene tyrosine protein kinase fyn antibody
      • Proto-oncogene c-Fyn antibody
      • Proto-oncogene Syn antibody
      • Protooncogene Syn antibody
      • SLK antibody
      • Src like kinase antibody
      • Src yes related novel gene antibody
      • Src-like kinase antibody
      • Src/yes related novel antibody
      • SYN antibody
      • Tyrosine kinase p59fyn T antibody
      • Tyrosine kinase p59fyn(T) antibody
      • Tyrosine-protein kinase Fyn antibody
      see all

    Images

    • Western blot - Anti-Fyn antibody (ab76934)
      Western blot - Anti-Fyn antibody (ab76934)
      Anti-Fyn antibody (ab76934) at 5 µg/ml + Immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size: 60 kDa
      Observed band size: 86 kDa
      why is the actual band size different from the predicted?



      This image was generated using the ascites version of the product.

    • Western blot - Anti-Fyn antibody (ab76934)
      Western blot - Anti-Fyn antibody (ab76934)
      All lanes : Anti-Fyn antibody (ab76934) at 5 µg/ml

      Lane 1 : Transfected 293T cell lysate
      Lane 2 : Untransfected 293T cell lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size: 60 kDa
      Observed band size: 60 kDa



      This image was generated using the ascites version of the product.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab76934? Please let us know so that we can cite the reference in this datasheet.

    ab76934 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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