Recombinant
RabMAb

Recombinant Anti-Fyn antibody [EPR19636] (ab184276)

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Overview

  • Product name

    Anti-Fyn antibody [EPR19636]
    See all Fyn primary antibodies

  • Description

    Rabbit monoclonal [EPR19636] to Fyn

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Mouse Fyn aa 1-100. The exact sequence is proprietary.
    Database link: P39688

  • Positive control

    • WB: Human brain lysate; Ramos, HEK-293, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; P0 mouse brain lysate; P0 rat brain lysate; Rat spleen lysate. IHC-P: Human tonsil and cerebral cortex tissues; Mouse spleen tissue; Rat testis tissue. Flow Cyt: RAW 264.7 cells. IP: P0 mouse brain lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/50.
IP 1/40.

Target

  • Function

    Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3.

  • Tissue specificity

    Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.

  • Cellular localization

    Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.
  • Information by UniProt

  • Database links

    see all

  • Form

    This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.

  • Alternative names

    • C syn protooncogene antibody
    • Fyn antibody
    • FYN oncogene related to SRC FGR YES antibody
    • FYN_HUMAN antibody
    • OKT3 induced calcium influx regulator antibody
    • P59 FYN antibody
    • p59-Fyn antibody
    • Protein tyrosine kinase fyn antibody
    • Proto oncogene tyrosine protein kinase fyn antibody
    • Proto-oncogene c-Fyn antibody
    • Proto-oncogene Syn antibody
    • Protooncogene Syn antibody
    • SLK antibody
    • Src like kinase antibody
    • Src yes related novel gene antibody
    • Src-like kinase antibody
    • Src/yes related novel antibody
    • SYN antibody
    • Tyrosine kinase p59fyn T antibody
    • Tyrosine kinase p59fyn(T) antibody
    • Tyrosine-protein kinase Fyn antibody
    see all

Images

  • Western blot - Anti-Fyn antibody [EPR19636] (ab184276)
    Western blot - Anti-Fyn antibody [EPR19636] (ab184276)
    All lanes : Anti-Fyn antibody [EPR19636] (ab184276) at 1/1000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 4 : P0 mouse brain lysate
    Lane 5 : P0 rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
    Lanes 2-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 61 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1 and 2: 3 minutes; Lanes 3,4 and 5: 8 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Fyn with ab184276 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on T cells of human tonsil (PMID: 10523617). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)

    Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling Fyn with ab184276 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human cerebral cortex (PMID: 7544314). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Fyn with ab184276 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on T cells of mouse spleen (PMID: 7544314, PMID:10523617). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling Fyn with ab184276 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on rat testis (PMID: 7544314). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Fyn antibody [EPR19636] (ab184276)
    Western blot - Anti-Fyn antibody [EPR19636] (ab184276)
    All lanes : Anti-Fyn antibody [EPR19636] (ab184276) at 1/1000 dilution

    Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
    Lane 4 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 61 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-Fyn antibody [EPR19636] (ab184276)
    Flow Cytometry - Anti-Fyn antibody [EPR19636] (ab184276)

    Flow cytometric analysis of 4% paraformaldehyde-fixed RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line labeling Fyn with ab184276 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-Fyn antibody [EPR19636] (ab184276)
    Immunoprecipitation - Anti-Fyn antibody [EPR19636] (ab184276)

    Fyn was immunoprecipitated from 0.35 mg of P0 mouse brain lysate with ab184276 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184276 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: P0 mouse brain lysate 10 µg (Input). 

    Lane 2: ab184276 IP in P0 mouse brain lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184276 in P0 mouse brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

References

ab184276 has not yet been referenced specifically in any publications.

Publishing research using ab184276? Please let us know so that we can cite the reference in this datasheet.

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