Recombinant
RabMAb

Recombinant Anti-Fyn antibody [EPR5500] - BSA and Azide free (ab240015)

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Overview

  • Product name

    Anti-Fyn antibody [EPR5500] - BSA and Azide free
    See all Fyn primary antibodies

  • Description

    Rabbit monoclonal [EPR5500] to Fyn - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Fyn aa 1-100. The exact sequence is proprietary.

  • General notes

    Ab240015 is the carrier-free version of ab125016. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240015 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3.

    • Tissue specificity

      Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.

    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.

    • Cellular localization

      Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.
    • Information by UniProt

    • Database links

      see all

    • Form

      This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.

    • Alternative names

      • C syn protooncogene antibody
      • Fyn antibody
      • FYN oncogene related to SRC FGR YES antibody
      • FYN_HUMAN antibody
      • OKT3 induced calcium influx regulator antibody
      • P59 FYN antibody
      • p59-Fyn antibody
      • Protein tyrosine kinase fyn antibody
      • Proto oncogene tyrosine protein kinase fyn antibody
      • Proto-oncogene c-Fyn antibody
      • Proto-oncogene Syn antibody
      • Protooncogene Syn antibody
      • SLK antibody
      • Src like kinase antibody
      • Src yes related novel gene antibody
      • Src-like kinase antibody
      • Src/yes related novel antibody
      • SYN antibody
      • Tyrosine kinase p59fyn T antibody
      • Tyrosine kinase p59fyn(T) antibody
      • Tyrosine-protein kinase Fyn antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [EPR5500] - BSA and Azide free (ab240015)
      Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [EPR5500] - BSA and Azide free (ab240015)

      Immunofluorescence staining of Neuro-2a cells with purified ab125016 at a working dilution of 1 in 500, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. ab7291 was used to stain tubulin, and this is shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom middle and right hand panels - for the negative controls, purified ab125016 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody at a dilution of 1/500.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab125016).

    References

    ab240015 has not yet been referenced specifically in any publications.

    Publishing research using ab240015? Please let us know so that we can cite the reference in this datasheet.

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