Recombinant Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512] (ab188319)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13512] to Fyn (phospho Y530) + Yes1 (phospho Y537)
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512] -
Description
Rabbit monoclonal [EPR13512] to Fyn (phospho Y530) + Yes1 (phospho Y537) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Lysate from HeLa cells treated with pervanadate; IP: HeLa cells treated with pervanadate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13512 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 59 kDa (predicted molecular weight: 61 kDa).
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IP |
1/30.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 59 kDa (predicted molecular weight: 61 kDa). |
IP
1/30. |
Target
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Cellular localization
Fyn: Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes. Yes1: Cytoplasm > cytosol. In epithelial cells infected with Neisseria gonorrhoeae, forms aggregates beneath bacterial microcolonies. -
Database links
- Entrez Gene: 2534 Human
- Entrez Gene: 7525 Human
- Omim: 137025 Human
- Omim: 164880 Human
- SwissProt: P06241 Human
- SwissProt: P07947 Human
- Unigene: 194148 Human
- Unigene: 390567 Human
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Form
Fyn: This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. Yes1: This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins m
Images
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All lanes : Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512] (ab188319) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50 nM pervanadate for 1 hour whole cell lysates
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50 nM pervanadate for 1 hour whole cell lysates. Then the membrane was incubated with phosphatase
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted? -
ab188319 (purified) at 1:30 dilution (2µg) immunoprecipitating Fyn (phospho Y530) + Yes1 (phospho Y537) in HeLa treated with 50nM Pervandate for 1h whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Pervandate for 1h whole cell lysate 10µg
Lane 2 (+): ab188319 & HeLa treated with 50nM Pervandate for 1h whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab188319 in HeLa treated with 50nM Pervandate for 1h whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
All lanes : Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512] (ab188319) at 1/20000 dilution (Unpurified)
Lane 1 : Lysate from HeLa cells treated with pervanadate
Lane 2 : Lysate from untreated HeLa cells
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 61 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab188319 has been referenced in 3 publications.
- Gray J et al. Heat shock protein amplification improves cerebellar myelination in the Npc1nih mouse model. EBioMedicine 86:104374 (2022). PubMed: 36455410
- Chen Y et al. IBSP, a potential recurrence biomarker, promotes the progression of colorectal cancer via Fyn/β-catenin signaling pathway. Cancer Med 10:4030-4045 (2021). PubMed: 33987980
- Hsia CW et al. Suppression of Human Platelet Activation via Integrin aIIbß3 Outside-In Independent Signal and Reduction of the Mortality in Pulmonary Thrombosis by Auraptene. Int J Mol Sci 20:N/A (2019). PubMed: 31717348