Recombinant
RabMAb

Recombinant Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] - BSA and Azide free (ab238975)

Overview

  • Product name

    Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] - BSA and Azide free
    See all Fyn (phospho Y530) + Yes (phospho Y537) primary antibodies
  • Description

    Rabbit monoclonal [EPR13512] to Fyn (phospho Y530) + Yes (phospho Y537) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Fyn (phospho Y530) + Yes (phospho Y537) aa 500 to the C-terminus. The exact sequence is proprietary.
    Database link: P06241

  • Positive control

    • WB: Lysate from HeLa cells treated with pervanadate; IP: HeLa cells treated with pervanadate.
  • General notes

    Ab238975 is the carrier-free version of ab188319. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238975 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 59 kDa (predicted molecular weight: 61 kDa).
IP Use at an assay dependent concentration.

Target

  • Database links

  • Alternative names

    • c syn protooncogene antibody
    • c yes antibody
    • Cellular yes 1 protein antibody
    • FYN oncogene related to SRC, FGR, YES antibody
    • HsT441 antibody
    • OKT3 induced calcium influx regulator antibody
    • p59 Fyn antibody
    • p61 Yes antibody
    • Proto oncogene c Fyn antibody
    • Proto oncogene c Yes antibody
    • Proto oncogene Syn antibody
    • Proto oncogene tyrosine protein kinase YES antibody
    • SLK antibody
    • Src like kinase antibody
    • src/yes related novel antibody
    • SYN antibody
    • Tyrosine kinase p59fyn(T) antibody
    • Tyrosine protein kinase Fyn antibody
    • Tyrosine protein kinase Yes antibody
    • v yes 1 Yamaguchi sarcoma viral oncogene homolog 1 antibody
    • Yamaguchi sarcoma oncogene antibody
    • YES1 antibody
    see all

Images

  • This image was made using ab188319 which is the same antibody as ab238975 with BSA and Azide
    ab188319 (purified) at 1:30 dilution (2µg) immunoprecipitating Fyn (phospho Y530) + Yes (phospho Y537) in HeLa treated with 50nM Pervandate for 1h whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Pervandate for 1h whole cell lysate 10µg
    Lane 2 (+): ab188319 & HeLa treated with 50nM Pervandate for 1h whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab188319 in HeLa treated with 50nM Pervandate for 1h whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

References

ab238975 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab238975.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up