Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13512] to Fyn (phospho Y530) + Yes (phospho Y537) - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] - BSA and Azide free
See all Fyn (phospho Y530) + Yes (phospho Y537) primary antibodies
DescriptionRabbit monoclonal [EPR13512] to Fyn (phospho Y530) + Yes (phospho Y537) - BSA and Azide free
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Fyn (phospho Y530) + Yes (phospho Y537) aa 500 to the C-terminus. The exact sequence is proprietary.
Database link: P06241
- WB: Lysate from HeLa cells treated with pervanadate; IP: HeLa cells treated with pervanadate.
Ab238975 is the carrier-free version of ab188319. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab238975 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab238975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 59 kDa (predicted molecular weight: 61 kDa).|
|IP||Use at an assay dependent concentration.|
- c syn protooncogene antibody
- c yes antibody
- Cellular yes 1 protein antibody
This image was made using ab188319 which is the same antibody as ab238975 with BSA and Azide
ab188319 (purified) at 1:30 dilution (2µg) immunoprecipitating Fyn (phospho Y530) + Yes (phospho Y537) in HeLa treated with 50nM Pervandate for 1h whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Pervandate for 1h whole cell lysate 10µg
Lane 2 (+): ab188319 & HeLa treated with 50nM Pervandate for 1h whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab188319 in HeLa treated with 50nM Pervandate for 1h whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238975 has not yet been referenced specifically in any publications.