Recombinant Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512] - BSA and Azide free (ab238975)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13512] to Fyn (phospho Y530) + Yes1 (phospho Y537) - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512] - BSA and Azide free -
Description
Rabbit monoclonal [EPR13512] to Fyn (phospho Y530) + Yes1 (phospho Y537) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Lysate from HeLa cells treated with pervanadate; IP: HeLa cells treated with pervanadate.
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General notes
ab238975 is the carrier-free version of ab188319.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13512 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 59 kDa (predicted molecular weight: 61 kDa).
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 59 kDa (predicted molecular weight: 61 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Cellular localization
Fyn: Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes. Yes1: Cytoplasm > cytosol. In epithelial cells infected with Neisseria gonorrhoeae, forms aggregates beneath bacterial microcolonies. -
Database links
- Entrez Gene: 2534 Human
- Entrez Gene: 7525 Human
- Omim: 137025 Human
- Omim: 164880 Human
- SwissProt: P06241 Human
- SwissProt: P07947 Human
- Unigene: 194148 Human
- Unigene: 390567 Human
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Form
Fyn: This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. Yes1: This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins m
Images
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All lanes : Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512] (ab188319) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50 nM pervanadate for 1 hour whole cell lysates
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50 nM pervanadate for 1 hour whole cell lysates. Then the membrane was incubated with phosphatase
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted?This image was made using ab188319 which is the same antibody as ab238975 with BSA and Azide
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This image was made using ab188319 which is the same antibody as ab238975 with BSA and Azide
ab188319 (purified) at 1:30 dilution (2µg) immunoprecipitating Fyn (phospho Y530) + Yes1 (phospho Y537) in HeLa treated with 50nM Pervandate for 1h whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Pervandate for 1h whole cell lysate 10µg
Lane 2 (+): ab188319 & HeLa treated with 50nM Pervandate for 1h whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab188319 in HeLa treated with 50nM Pervandate for 1h whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
All lanes : Anti-Fyn (phospho Y530) + Yes1 (phospho Y537) antibody [EPR13512] (ab188319) at 1/20000 dilution (Unpurified)
Lane 1 : Lysate from HeLa cells treated with pervanadate
Lane 2 : Lysate from untreated HeLa cells
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 61 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted?This image was made using ab188319 which is the same antibody as ab238975 with BSA and Azide
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab238975 has not yet been referenced specifically in any publications.